Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5131267..5131869 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NML25_RS24630 Protein ID WP_000897305.1
Coordinates 5131267..5131578 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NML25_RS24635 Protein ID WP_000356395.1
Coordinates 5131579..5131869 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS24605 (5126309) 5126309..5126746 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NML25_RS24610 (5126791) 5126791..5127732 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NML25_RS24615 (5128148) 5128148..5129035 + 888 Protein_4798 hypothetical protein -
NML25_RS24620 (5129048) 5129048..5129962 + 915 WP_233304549.1 transposase -
NML25_RS24625 (5130130) 5130130..5131038 - 909 WP_001550353.1 alpha/beta hydrolase -
NML25_RS24630 (5131267) 5131267..5131578 + 312 WP_000897305.1 hypothetical protein Toxin
NML25_RS24635 (5131579) 5131579..5131869 + 291 WP_000356395.1 NadS family protein Antitoxin
NML25_RS24640 (5131954) 5131954..5132175 + 222 WP_001550354.1 hypothetical protein -
NML25_RS24645 (5132227) 5132227..5132505 + 279 WP_001315112.1 hypothetical protein -
NML25_RS24650 (5132924) 5132924..5133142 + 219 WP_001295676.1 CopG family transcriptional regulator -
NML25_RS24655 (5133361) 5133361..5133603 + 243 WP_001309881.1 CopG family transcriptional regulator -
NML25_RS24660 (5133785) 5133785..5134714 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
NML25_RS24665 (5134711) 5134711..5135346 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NML25_RS24670 (5135343) 5135343..5136245 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T251564 WP_000897305.1 NZ_CP101222:5131267-5131578 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT251564 WP_000356395.1 NZ_CP101222:5131579-5131869 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References