Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4297668..4298467 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NML25_RS20660 Protein ID WP_000347270.1
Coordinates 4298003..4298467 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NML25_RS20655 Protein ID WP_001551693.1
Coordinates 4297668..4298003 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS20640 (4293453) 4293453..4294223 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
NML25_RS20645 (4294239) 4294239..4295573 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NML25_RS20650 (4295948) 4295948..4297519 + 1572 WP_001273738.1 galactarate dehydratase -
NML25_RS20655 (4297668) 4297668..4298003 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NML25_RS20660 (4298003) 4298003..4298467 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NML25_RS20665 (4298522) 4298522..4299331 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NML25_RS20670 (4299580) 4299580..4300860 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NML25_RS20675 (4300883) 4300883..4301356 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NML25_RS20680 (4301367) 4301367..4302146 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NML25_RS20685 (4302136) 4302136..4303014 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NML25_RS20690 (4303032) 4303032..4303466 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4288906..4298467 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T251562 WP_000347270.1 NZ_CP101222:4298003-4298467 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT251562 WP_001551693.1 NZ_CP101222:4297668-4298003 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References