Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3729493..3730220 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NML25_RS17860 Protein ID WP_000547555.1
Coordinates 3729909..3730220 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NML25_RS17855 Protein ID WP_000126294.1
Coordinates 3729493..3729912 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS17835 (3724578) 3724578..3725105 - 528 WP_001078777.1 electron transport protein HydN -
NML25_RS17840 (3725254) 3725254..3726264 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NML25_RS17845 (3726524) 3726524..3727981 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NML25_RS17850 (3727990) 3727990..3729414 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NML25_RS17855 (3729493) 3729493..3729912 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NML25_RS17860 (3729909) 3729909..3730220 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NML25_RS17865 (3730387) 3730387..3730857 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NML25_RS17870 (3730850) 3730850..3731260 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NML25_RS17875 (3731257) 3731257..3732024 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NML25_RS17880 (3732024) 3732024..3732566 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NML25_RS17885 (3732576) 3732576..3734285 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T251557 WP_000547555.1 NZ_CP101222:c3730220-3729909 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT251557 WP_000126294.1 NZ_CP101222:c3729912-3729493 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References