Detailed information of TA system TA025155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4010901..4011484
• Replicon: chromosome
• Accession: NC_015224
• Organism: Yersinia enterocolitica subsp. palearctica 105.5R(r)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: YE105_RS18245
• Protein ID: WP_005162526.1
• Coordinates: 4010901..4011179 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: YE105_RS18250
• Protein ID: WP_005162529.1
• Coordinates: 4011179..4011484 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YE105_RS18235	4006382..4007329	-	948	WP_005162520.1	trehalose operon repressor TreR	-
YE105_RS18240	4008101..4010821	+	2721	WP_005162523.1	magnesium-translocating P-type ATPase	-
YE105_RS18245	4010901..4011179	+	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
YE105_RS18250	4011179..4011484	+	306	WP_005162529.1	HigA family addiction module antidote protein	Antitoxin
YE105_RS18255	4011556..4013484	-	1929	WP_013650488.1	BglG family transcription antiterminator	-
YE105_RS18260	4013557..4014798	-	1242	WP_013650489.1	lactonase family protein	-
YE105_RS18265	4014928..4015668	-	741	WP_013650490.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T25155 WP_005162526.1 NC_015224:4010901-4011179 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T25155 NC_015224:4010901-4011179 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
ATGATAAAAAGTTGGAAACACAAAGGACTGCAACGCTTCTTTGAAACAGGTTCTGCCGCGGGCATTAATCCCCAGCATGT
TAAGCGAATTCGAGACCGCTTGCGATTTATCGACCTGGCGGAAACGATTGAGGATGTCAATGTTGCCGGATACAAGCTCC
ATCCGTTACAAGGTGAAAGAGAAGGGATCTGGTCAGTAACAGTAAGCGGAAATTGGCGCATTACATTCGAGTTTACCGCT
GGAGATGCGTTTATTCTTAACTACGAGGACTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10934.77 Da
• Isoelectric Point: 6.7158

>AT25155 WP_005162529.1 NC_015224:4011179-4011484 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVACKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT25155 NC_015224:4011179-4011484 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
ATGAAAATGCATAATCCCCCACATCCAGGGGAAATGATTGAAGGAATCCTCGAAGATTTGGGTCTAGGGATAAGGGAGTT
TGCCAGGGCATTAAACGTGTCACCTTCAACGGTACAACGGCTGGTTGCCTGTAAGGCGGCAATTTCACCGGAAATGGCGG
TAAAACTGGCCGCGGTTCTGGGAAGTACCCCAGCAATGTGGCTCCGCCTCCAGGCCGCTTATGACCTGGGAAAAGCAGAA
GAGAATGTTGATGTATCCAATCTGACTAAACTGTACAAACCGGATCCAATATCGCTGCATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5