Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3633734..3634413 Replicon chromosome
Accession NZ_CP101210
Organism Escherichia coli strain EC21Z-147

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag NML26_RS17920 Protein ID WP_000057523.1
Coordinates 3634111..3634413 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NML26_RS17915 Protein ID WP_000806442.1
Coordinates 3633734..3634075 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML26_RS17905 (3629977) 3629977..3630909 - 933 WP_000883024.1 glutaminase A -
NML26_RS17910 (3631172) 3631172..3633676 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
NML26_RS17915 (3633734) 3633734..3634075 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NML26_RS17920 (3634111) 3634111..3634413 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NML26_RS17925 (3634546) 3634546..3635340 + 795 WP_000365161.1 TraB/GumN family protein -
NML26_RS17930 (3635544) 3635544..3636023 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NML26_RS17935 (3636060) 3636060..3637712 - 1653 Protein_3512 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
NML26_RS17940 (3637930) 3637930..3639150 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T251522 WP_000057523.1 NZ_CP101210:c3634413-3634111 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT251522 WP_000806442.1 NZ_CP101210:c3634075-3633734 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References