Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1197763..1198490 Replicon chromosome
Accession NZ_CP101197
Organism Escherichia coli strain EC21Z-151

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NML27_RS05810 Protein ID WP_000547555.1
Coordinates 1197763..1198074 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NML27_RS05815 Protein ID WP_000126294.1
Coordinates 1198071..1198490 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML27_RS05785 (1193698) 1193698..1195407 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NML27_RS05790 (1195417) 1195417..1195959 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NML27_RS05795 (1195959) 1195959..1196726 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NML27_RS05800 (1196723) 1196723..1197133 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NML27_RS05805 (1197126) 1197126..1197596 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NML27_RS05810 (1197763) 1197763..1198074 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NML27_RS05815 (1198071) 1198071..1198490 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NML27_RS05820 (1198569) 1198569..1199993 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NML27_RS05825 (1200002) 1200002..1201459 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NML27_RS05830 (1201719) 1201719..1202729 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NML27_RS05835 (1202878) 1202878..1203405 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T251476 WP_000547555.1 NZ_CP101197:1197763-1198074 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT251476 WP_000126294.1 NZ_CP101197:1198071-1198490 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References