Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629516..630315 Replicon chromosome
Accession NZ_CP101197
Organism Escherichia coli strain EC21Z-151

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NML27_RS03010 Protein ID WP_000347270.1
Coordinates 629516..629980 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NML27_RS03015 Protein ID WP_001551693.1
Coordinates 629980..630315 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML27_RS02980 (624517) 624517..624951 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NML27_RS02985 (624969) 624969..625847 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NML27_RS02990 (625837) 625837..626616 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NML27_RS02995 (626627) 626627..627100 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NML27_RS03000 (627123) 627123..628403 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NML27_RS03005 (628652) 628652..629461 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NML27_RS03010 (629516) 629516..629980 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NML27_RS03015 (629980) 629980..630315 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NML27_RS03020 (630464) 630464..632035 - 1572 WP_001273738.1 galactarate dehydratase -
NML27_RS03025 (632410) 632410..633744 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NML27_RS03030 (633760) 633760..634530 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T251471 WP_000347270.1 NZ_CP101197:c629980-629516 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT251471 WP_001551693.1 NZ_CP101197:c630315-629980 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References