Detailed information of TA system TA025145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 2090243..2090788
• Replicon: chromosome
• Accession: NC_015224
• Organism: Yersinia enterocolitica subsp. palearctica 105.5R(r)

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: YE105_RS09435
• Protein ID: WP_013649743.1
• Coordinates: 2090510..2090788 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A7T9Y052
• Locus tag: YE105_RS09430
• Protein ID: WP_005161466.1
• Coordinates: 2090243..2090503 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YE105_RS09400	2085404..2086579	-	1176	WP_013649737.1	phage tail sheath protein	-
YE105_RS09405	2086889..2087074	-	186	WP_013649738.1	hypothetical protein	-
YE105_RS22400	2087208..2087346	-	139	Protein_1797	ash family protein	-
YE105_RS09410	2087638..2088522	-	885	WP_072077075.1	Rpn family recombination-promoting nuclease/putative transposase	-
YE105_RS23140	2088728..2088904	-	177	WP_013649740.1	hypothetical protein	-
YE105_RS09415	2089346..2089666	-	321	WP_013649741.1	hypothetical protein	-
YE105_RS09420	2089656..2089862	-	207	WP_013649742.1	hypothetical protein	-
YE105_RS23145	2090004..2090171	+	168	WP_005161469.1	hypothetical protein	-
YE105_RS09430	2090243..2090503	+	261	WP_005161466.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
YE105_RS09435	2090510..2090788	+	279	WP_013649743.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
YE105_RS21545	2090841..2091453	-	613	Protein_1805	tail fiber assembly protein	-
YE105_RS09450	2091453..2092745	-	1293	WP_013649744.1	phage tail protein	-
YE105_RS23080	2092718..2092999	-	282	WP_013649745.1	phage tail protein	-
YE105_RS09460	2092992..2093537	-	546	WP_013649746.1	phage tail protein I	-
YE105_RS09465	2093530..2094438	-	909	WP_013649747.1	baseplate assembly protein	-
YE105_RS09470	2094438..2094794	-	357	WP_005161449.1	GPW/gp25 family protein	-
YE105_RS09475	2094791..2095426	-	636	WP_013649748.1	phage baseplate assembly protein V	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	pilW	2078778..2122044	43266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10930.63 Da
• Isoelectric Point: 9.8470

>T25145 WP_013649743.1 NC_015224:2090510-2090788 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
MTKQREIEYSGQFQKDVKKAQKRHKDINKLKTIMALLIDDKLPLPVIYKDHQLQGNYKGYRDAHIEPDWLIIYKITDDLL
RFGRTGSHSDLF

Nucleotide

• Length: 279 bp

>T25145 NC_015224:2090510-2090788 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
ATGACTAAACAAAGGGAAATTGAATATTCAGGTCAGTTCCAGAAAGATGTGAAAAAAGCTCAGAAGCGCCATAAAGATAT
AAATAAACTTAAAACTATTATGGCGCTGCTAATTGACGATAAATTACCCTTGCCTGTTATATACAAGGATCATCAATTAC
AAGGTAATTACAAAGGTTACAGGGATGCGCATATAGAGCCAGACTGGCTTATCATCTACAAGATTACTGATGATTTGCTT
CGCTTCGGAAGGACAGGAAGCCATTCTGATCTATTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9277.75 Da
• Isoelectric Point: 7.0134

>AT25145 WP_005161466.1 NC_015224:2090243-2090503 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
MAANAFVRARIDETLKNEAAAVLAGMGLTVSDLVRITLTKVAREKALPFDLRIPNELTAKTIANSEKGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT25145 NC_015224:2090243-2090503 [Yersinia enterocolitica subsp. palearctica 105.5R(r)]
ATGGCTGCAAATGCTTTTGTTCGTGCTCGTATAGATGAAACCCTAAAAAATGAAGCCGCTGCTGTGCTTGCTGGCATGGG
TTTAACCGTATCCGATCTGGTGCGGATAACCTTAACCAAGGTCGCCCGAGAAAAAGCGCTTCCGTTCGATTTGCGTATTC
CTAACGAACTTACCGCTAAAACTATTGCCAACAGCGAGAAAGGTGTCGATGTCCACAAAGCAAAGGATGCCGATGATCTG
TTTGATAAATTAGGTATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7T9Y052