Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 213542..214170 Replicon chromosome
Accession NZ_CP100753
Organism Serratia marcescens strain SASK1000

Toxin (Protein)


Gene name higB Uniprot ID A0A6M5HY34
Locus tag NLX77_RS01020 Protein ID WP_048325546.1
Coordinates 213880..214170 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag NLX77_RS01015 Protein ID WP_015379150.1
Coordinates 213542..213865 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLX77_RS00990 (NLX77_00990) 208554..209789 - 1236 WP_004937146.1 serine/threonine transporter SstT -
NLX77_RS00995 (NLX77_00995) 210052..211017 - 966 WP_049241556.1 TerC family protein -
NLX77_RS01000 (NLX77_01000) 211273..212004 - 732 WP_015379152.1 glutamine amidotransferase -
NLX77_RS01005 (NLX77_01005) 212006..212986 - 981 WP_004937152.1 Gfo/Idh/MocA family oxidoreductase -
NLX77_RS01010 (NLX77_01010) 213037..213549 - 513 WP_016930176.1 M48 family metallopeptidase -
NLX77_RS01015 (NLX77_01015) 213542..213865 - 324 WP_015379150.1 HigA family addiction module antitoxin Antitoxin
NLX77_RS01020 (NLX77_01020) 213880..214170 - 291 WP_048325546.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NLX77_RS01025 (NLX77_01025) 214401..215540 + 1140 WP_015379148.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NLX77_RS01030 (NLX77_01030) 215735..217756 - 2022 WP_118891652.1 NADPH-dependent 2,4-dienoyl-CoA reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11627.31 Da        Isoelectric Point: 10.2325

>T251355 WP_048325546.1 NZ_CP100753:c214170-213880 [Serratia marcescens]
MIKSFRDRYLEQFYLEGKRSRLIPGVLERQLARKLDILAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT251355 WP_015379150.1 NZ_CP100753:c213865-213542 [Serratia marcescens]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M5HY34

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References