Detailed information of TA system TA025120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3299418..3299988
• Replicon: chromosome
• Accession: NC_015125
• Organism: Microbacterium testaceum StLB037

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MTES_RS18970
• Protein ID: WP_013586125.1
• Coordinates: 3299418..3299699 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MTES_RS15005
• Protein ID: WP_013586126.1
• Coordinates: 3299686..3299988 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTES_RS14980	3294607..3295122	+	516	WP_013586121.1	hypothetical protein	-
MTES_RS14985	3296118..3297089	-	972	WP_013586122.1	IS481 family transposase	-
MTES_RS18965	3297234..3298146	+	913	WP_099046929.1	IS5 family transposase	-
MTES_RS15000	3298880..3299146	-	267	WP_013586124.1	Txe/YoeB family addiction module toxin	-
MTES_RS18970	3299418..3299699	+	282	WP_013586125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MTES_RS15005	3299686..3299988	+	303	WP_013586126.1	HigA family addiction module antidote protein	Antitoxin
MTES_RS15010	3300153..3300596	-	444	WP_013586127.1	hypothetical protein	-
MTES_RS15015	3300581..3301327	-	747	WP_148272896.1	hypothetical protein	-
MTES_RS15020	3301760..3301972	+	213	WP_013586129.1	hypothetical protein	-
MTES_RS15025	3302003..3302365	+	363	WP_013586130.1	DUF4440 domain-containing protein	-
MTES_RS15030	3302368..3303321	-	954	WP_013586131.1	ADP-ribosylglycohydrolase family protein	-
MTES_RS15035	3303415..3304155	+	741	WP_013586132.1	type IV secretory pathway, VirD4 components	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3289814..3302365	12551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-97)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10763.17 Da
• Isoelectric Point: 9.2001

>T25120 WP_013586125.1 NC_015125:3299418-3299699 [Microbacterium testaceum StLB037]
VIRSFGTKETERLWRRERVPSIDPRIHSTALRKLRQVGSAESLDDLRVPTGNRLEALKGARAGQHSIRINDQWRICFVWT
DAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T25120 NC_015125:3299418-3299699 [Microbacterium testaceum StLB037]
GTGATCAGGTCGTTCGGGACCAAGGAGACCGAGCGGCTGTGGCGTCGTGAACGCGTGCCCTCGATCGATCCCCGGATCCA
TTCGACGGCGTTGCGAAAGCTGCGTCAGGTGGGGTCTGCCGAGTCTCTGGACGATCTTCGGGTTCCAACAGGAAACCGGC
TCGAGGCTCTCAAGGGCGCTCGAGCAGGGCAACACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GACGCTGGGCCAGAGGAGGTGGAGATCGTTGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10929.49 Da
• Isoelectric Point: 4.7318

>AT25120 WP_013586126.1 NC_015125:3299686-3299988 [Microbacterium testaceum StLB037]
MTITDKIPPVHPGEVLLEDFINGLGITQNKLAVAIGVPPRRINEIVHGARGISADTALRLSRYFGTSAEFWINLQSYYEL
DRAEDLAGEQIAAITPLQVA

Nucleotide

• Length: 303 bp

>AT25120 NC_015125:3299686-3299988 [Microbacterium testaceum StLB037]
TTGACTATCACTGACAAGATTCCCCCGGTCCACCCCGGTGAAGTTCTTCTGGAGGACTTCATCAACGGGCTGGGGATCAC
GCAGAACAAGCTCGCTGTCGCCATCGGGGTTCCGCCACGGCGGATCAACGAGATCGTGCACGGCGCTCGCGGGATCAGCG
CGGACACCGCGCTTCGATTGTCGAGGTACTTCGGTACCTCGGCGGAGTTCTGGATCAATCTCCAGAGCTACTACGAACTC
GATCGTGCCGAAGATCTCGCGGGGGAGCAGATCGCCGCGATCACACCCCTCCAAGTCGCGTGA