Detailed information of TA system TA025115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1338053..1338700
• Replicon: chromosome
• Accession: NC_015064
• Organism: Granulicella tundricola MP5ACTX9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E8X3L3
• Locus tag: ACIX9_RS05715
• Protein ID: WP_013579527.1
• Coordinates: 1338407..1338700 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACIX9_RS05710
• Protein ID: WP_013579526.1
• Coordinates: 1338053..1338388 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX9_RS05690	1333287..1334630	+	1344	WP_157477813.1	methyltransferase domain-containing protein	-
ACIX9_RS05695	1334648..1335481	-	834	WP_013579523.1	PASTA domain-containing protein	-
ACIX9_RS05700	1335599..1336729	-	1131	WP_157477815.1	SH3 domain-containing protein	-
ACIX9_RS05705	1336836..1337987	-	1152	WP_013579525.1	beta-propeller fold lactonase family protein	-
ACIX9_RS05710	1338053..1338388	-	336	WP_013579526.1	HigA family addiction module antidote protein	Antitoxin
ACIX9_RS05715	1338407..1338700	-	294	WP_013579527.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIX9_RS05720	1338822..1339355	+	534	WP_013579528.1	hypothetical protein	-
ACIX9_RS05725	1339440..1340975	-	1536	WP_013579529.1	L-aspartate oxidase	-
ACIX9_RS05730	1341049..1342566	-	1518	WP_013579530.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11609.14 Da
• Isoelectric Point: 6.4619

>T25115 WP_013579527.1 NC_015064:c1338700-1338407 [Granulicella tundricola MP5ACTX9]
MIETWRESWLREFFVNDVYSKKIPADLRDRLFRKLQLLDDASTDADLRVPPSNRFEHLSGKLEGFCSIRVNDKWRLIFQW
SEGKATELCLDDHSYRS

Nucleotide

• Length: 294 bp

>T25115 NC_015064:c1338700-1338407 [Granulicella tundricola MP5ACTX9]
ATGATTGAAACTTGGCGGGAGAGTTGGCTTCGCGAATTCTTCGTGAACGATGTGTATTCGAAGAAGATTCCGGCTGATCT
GCGCGACAGGCTGTTTCGTAAGTTGCAGCTTCTTGACGATGCGAGCACCGATGCCGATCTTAGAGTGCCTCCGAGTAATC
GGTTTGAACATCTGAGTGGAAAGCTGGAAGGCTTCTGCTCGATCCGGGTCAATGACAAGTGGCGATTGATCTTCCAGTGG
AGCGAAGGTAAGGCGACCGAGCTGTGTTTAGATGATCATTCTTATCGTTCTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12511.57 Da
• Isoelectric Point: 11.2099

>AT25115 WP_013579526.1 NC_015064:c1338388-1338053 [Granulicella tundricola MP5ACTX9]
MLMTKRKPVGVGEMLVEEFLEPLGISQTELAARAGLPRKHVNELCRGRRSVTADTALILSRVFGNSADFWLNTQRRTDLW
EALNTPKRLLRIERAKPISDRVSEKRVAVAG

Nucleotide

• Length: 336 bp

>AT25115 NC_015064:c1338388-1338053 [Granulicella tundricola MP5ACTX9]
ATGTTGATGACGAAGAGAAAGCCCGTTGGTGTTGGGGAGATGCTGGTGGAAGAGTTTCTGGAGCCGCTGGGGATCTCGCA
GACGGAGTTGGCGGCGCGGGCGGGGTTGCCGCGCAAACATGTCAATGAGCTATGCCGGGGACGCCGCTCCGTGACCGCGG
ATACGGCGTTGATCCTGAGCCGGGTGTTTGGCAACTCGGCCGATTTCTGGCTGAACACGCAGCGGCGAACCGATCTTTGG
GAAGCGCTCAATACCCCTAAGCGGCTGCTACGGATTGAACGAGCGAAGCCGATTTCCGACAGGGTGTCTGAGAAGAGGGT
TGCAGTGGCGGGATAA

Structures

Toxin

• AlphaFold DB: E8X3L3