Detailed information of TA system TA025112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 116114..116622
• Replicon: plasmid pRAHAQ02
• Accession: NC_015063
• Organism: Rahnella sp. Y9602

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H8P157
• Locus tag: RAHAQ_RS26475
• Protein ID: WP_013578404.1
• Coordinates: 116114..116380 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H8P156
• Locus tag: RAHAQ_RS25660
• Protein ID: WP_013578405.1
• Coordinates: 116377..116622 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RAHAQ_RS25640	111368..111814	+	447	WP_013578401.1	hypothetical protein	-
RAHAQ_RS25645	111927..112484	+	558	WP_193784878.1	hypothetical protein	-
RAHAQ_RS26470	113898..114033	-	136	Protein_108	transposase	-
RAHAQ_RS25655	114127..114660	-	534	WP_013578402.1	thioredoxin fold domain-containing protein	-
RAHAQ_RS26615	115084..115302	-	219	WP_148232237.1	hypothetical protein	-
RAHAQ_RS26960	115429..115792	-	364	Protein_111	GGDEF domain-containing protein	-
RAHAQ_RS26475	116114..116380	-	267	WP_013578404.1	Txe/YoeB family addiction module toxin	Toxin
RAHAQ_RS25660	116377..116622	-	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RAHAQ_RS25665	116953..118002	+	1050	WP_041689277.1	tyrosine-type recombinase/integrase	-
RAHAQ_RS26480	118094..118573	-	480	WP_013578407.1	GNAT family N-acetyltransferase	-
RAHAQ_RS25670	118570..118830	-	261	WP_013578408.1	DUF1778 domain-containing protein	-
RAHAQ_RS26620	118923..119165	+	243	Protein_117	serine recombinase	-
RAHAQ_RS25675	119724..120332	-	609	WP_013578409.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133486	133486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10490.04 Da
• Isoelectric Point: 10.0392

>T25112 WP_013578404.1 NC_015063:c116380-116114 [Rahnella sp. Y9602]
MKTRFTKRGAADYQYWQGSNAKIFERIRILIHAIHADPLGGIGKPERLRHYKNPALYSRRIDREHRLVYSMVNDELTIIA
CRYHYDDV

Nucleotide

• Length: 267 bp

>T25112 NC_015063:c116380-116114 [Rahnella sp. Y9602]
ATGAAAACGCGATTCACTAAGAGAGGGGCAGCCGATTATCAATACTGGCAGGGAAGTAACGCCAAAATATTCGAGCGCAT
CCGTATACTGATACATGCGATTCACGCCGACCCACTGGGCGGTATTGGGAAGCCAGAACGGTTGCGCCATTATAAAAATC
CGGCACTTTATTCCCGGCGCATAGACAGAGAACACCGACTTGTTTACAGCATGGTAAATGATGAATTAACTATAATTGCC
TGCCGGTATCATTATGACGATGTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9027.06 Da
• Isoelectric Point: 4.1947

>AT25112 WP_013578405.1 NC_015063:c116622-116377 [Rahnella sp. Y9602]
MHSLTFTDARKHFAETMQQVSDNAEPVRILRRDAPDVVLIDAAEYDAILETLYLFSNPANAAHINASLAELDRGDTVEIE
V

Nucleotide

• Length: 246 bp

>AT25112 NC_015063:c116622-116377 [Rahnella sp. Y9602]
ATGCATTCATTAACGTTTACTGATGCCCGTAAGCATTTTGCTGAGACAATGCAGCAGGTTAGCGACAATGCCGAGCCCGT
GCGTATTCTGCGACGTGATGCTCCTGATGTTGTACTGATTGACGCCGCCGAATATGACGCTATTTTAGAAACACTATATT
TATTCAGCAATCCTGCGAACGCTGCACATATTAACGCATCGCTGGCTGAGTTAGATCGCGGTGACACTGTCGAAATTGAA
GTATGA

Structures

Toxin

• AlphaFold DB: H8P157

Antitoxin

• AlphaFold DB: H8P156