Detailed information of TA system TA025098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2686620..2687305
• Replicon: chromosome
• Accession: NC_015061
• Organism: Rahnella sp. Y9602

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H8NS19
• Locus tag: RAHAQ_RS12145
• Protein ID: WP_013575753.1
• Coordinates: 2686620..2686934 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RAHAQ_RS12150
• Protein ID: WP_013575754.1
• Coordinates: 2686988..2687305 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RAHAQ_RS12135	2683462..2684481	+	1020	WP_013575750.1	transporter	-
RAHAQ_RS12140	2684503..2686206	-	1704	WP_013575751.1	chloride channel protein	-
RAHAQ_RS26775	2686213..2686446	-	234	WP_013575752.1	hypothetical protein	-
RAHAQ_RS12145	2686620..2686934	+	315	WP_013575753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RAHAQ_RS12150	2686988..2687305	+	318	WP_013575754.1	HigA family addiction module antidote protein	Antitoxin
RAHAQ_RS12155	2687420..2688997	-	1578	WP_013575755.1	ABC transporter substrate-binding protein	-
RAHAQ_RS12160	2689140..2689817	-	678	WP_013575756.1	ATP-binding cassette domain-containing protein	-
RAHAQ_RS12165	2689810..2690694	-	885	WP_013575757.1	ABC transporter ATP-binding protein	-
RAHAQ_RS12170	2690684..2691562	-	879	WP_013575758.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12595.39 Da
• Isoelectric Point: 9.2702

>T25098 WP_013575753.1 NC_015061:2686620-2686934 [Rahnella sp. Y9602]
MNDMLRISSFRDQWLEDFFHFATPHRKIPWTLATALERKLDMINAAMDYRDLLWPPGNRYEDLAPPLKGFSSIRVNEQYR
LIFYWREGKAHGLYLDPHGYRSYR

Nucleotide

• Length: 315 bp

>T25098 NC_015061:2686620-2686934 [Rahnella sp. Y9602]
ATGAACGATATGCTCAGGATAAGCAGTTTCCGCGATCAATGGCTGGAAGACTTTTTCCACTTCGCCACTCCCCATCGCAA
AATACCCTGGACGCTGGCAACTGCTCTGGAAAGGAAGCTGGATATGATCAATGCCGCAATGGATTATCGCGATTTACTCT
GGCCCCCGGGCAATCGCTATGAAGATCTGGCCCCCCCGTTAAAGGGATTTTCATCGATCAGGGTGAATGAGCAGTACCGT
CTGATTTTTTACTGGCGGGAAGGAAAAGCACATGGCCTGTATTTAGATCCCCACGGTTACAGGAGTTACCGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12143.04 Da
• Isoelectric Point: 8.6582

>AT25098 WP_013575754.1 NC_015061:2686988-2687305 [Rahnella sp. Y9602]
MAVTTRKPATVGDILLYEFLEPAGLRINDLADMLNVHRNTISALVNNNRRLTVDIAFRLAKTFETSVEFWLNLQTNVDIW
EVRNNPRLMDDINKVAHLKDFMKKS

Nucleotide

• Length: 318 bp

>AT25098 NC_015061:2686988-2687305 [Rahnella sp. Y9602]
ATGGCTGTGACCACACGTAAACCCGCTACTGTTGGTGATATTTTACTTTACGAATTTCTCGAACCTGCAGGCCTGCGGAT
CAACGATCTCGCCGACATGCTGAATGTTCACCGCAATACCATCAGCGCACTGGTGAATAACAACCGCAGACTGACCGTTG
ATATTGCCTTCCGGCTGGCAAAGACATTTGAGACCTCTGTCGAGTTTTGGTTAAACCTGCAAACCAATGTCGATATTTGG
GAAGTGCGCAATAACCCGCGCCTGATGGATGATATTAACAAAGTCGCCCATCTGAAAGATTTTATGAAAAAGTCATGA

Structures

Toxin

• AlphaFold DB: H8NS19