250904

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5272709..5273310 Replicon chromosome
Accession NZ_CP100652
Organism Pseudomonas putida strain HSM-C2

Toxin (Protein)


Gene name higB Uniprot ID A0A379KEL1
Locus tag NL778_RS24075 Protein ID WP_046788307.1
Coordinates 5272996..5273310 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL778_RS24070 Protein ID WP_046788286.1
Coordinates 5272709..5272996 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL778_RS24060 (NL778_24060) 5269546..5270463 + 918 WP_254466764.1 sce7725 family protein -
NL778_RS24065 (NL778_24065) 5270483..5272237 + 1755 WP_204919154.1 anti-phage dCTP deaminase -
NL778_RS24070 (NL778_24070) 5272709..5272996 - 288 WP_046788286.1 helix-turn-helix domain-containing protein Antitoxin
NL778_RS24075 (NL778_24075) 5272996..5273310 - 315 WP_046788307.1 hypothetical protein Toxin
NL778_RS24080 (NL778_24080) 5273529..5274176 + 648 WP_254466766.1 hypothetical protein -
NL778_RS24085 (NL778_24085) 5274577..5276238 + 1662 WP_254466767.1 FMN-binding glutamate synthase family protein -
NL778_RS24090 (NL778_24090) 5276356..5277675 - 1320 WP_012316697.1 OprD family porin -
NL778_RS24095 (NL778_24095) 5278105..5278302 - 198 WP_012316698.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11831.73 Da        Isoelectric Point: 10.1456

>T250904 WP_046788307.1 NZ_CP100652:c5273310-5272996 [Pseudomonas putida]
MIFIETPIFTQDVKEHLGDDEYRELQSYLAGQPIAGDLIEGTGGLRKIRWSAKGKGKSGGVRVIYYHVSSAYQIRMILIY
RKGIMDTLTDKQKAQLRAINKGWK

Download         Length: 315 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10804.25 Da        Isoelectric Point: 7.3222

>AT250904 WP_046788286.1 NZ_CP100652:c5272996-5272709 [Pseudomonas putida]
MEKNLFDRLVESMTQMDEIDRGERQPSREFHVDAVQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A379KEL1

Antitoxin

Source ID Structure

References