Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4475084..4475686 Replicon chromosome
Accession NZ_CP100544
Organism Escherichia coli strain LH09-a

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NLZ06_RS21360 Protein ID WP_000897305.1
Coordinates 4475375..4475686 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NLZ06_RS21355 Protein ID WP_000356397.1
Coordinates 4475084..4475374 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ06_RS21330 (4470631) 4470631..4471266 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NLZ06_RS21335 (4471263) 4471263..4472192 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
NLZ06_RS21340 (4472591) 4472591..4473571 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
NLZ06_RS21345 (4473800) 4473800..4474042 - 243 WP_001086388.1 protein YiiF -
NLZ06_RS21350 (4474261) 4474261..4474479 - 219 WP_001295676.1 CopG family transcriptional regulator -
NLZ06_RS21355 (4475084) 4475084..4475374 - 291 WP_000356397.1 NadS family protein Antitoxin
NLZ06_RS21360 (4475375) 4475375..4475686 - 312 WP_000897305.1 hypothetical protein Toxin
NLZ06_RS21365 (4475915) 4475915..4476823 + 909 WP_001162704.1 alpha/beta hydrolase -
NLZ06_RS21370 (4476887) 4476887..4477828 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NLZ06_RS21375 (4477873) 4477873..4478310 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NLZ06_RS21380 (4478307) 4478307..4479179 - 873 WP_000920762.1 virulence factor BrkB family protein -
NLZ06_RS21385 (4479173) 4479173..4479772 - 600 WP_001315111.1 glucose-1-phosphatase -
NLZ06_RS21390 (4479871) 4479871..4480656 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4472591..4473571 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T250839 WP_000897305.1 NZ_CP100544:c4475686-4475375 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT250839 WP_000356397.1 NZ_CP100544:c4475374-4475084 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References