Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3847132..3847826 Replicon chromosome
Accession NZ_CP100530
Organism Escherichia coli strain LH10-b-1

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag NLZ08_RS19070 Protein ID WP_001263493.1
Coordinates 3847132..3847530 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NLZ08_RS19075 Protein ID WP_000554757.1
Coordinates 3847533..3847826 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3842792) 3842792..3842872 - 81 NuclAT_13 - -
- (3842792) 3842792..3842872 - 81 NuclAT_13 - -
- (3842792) 3842792..3842872 - 81 NuclAT_13 - -
- (3842792) 3842792..3842872 - 81 NuclAT_13 - -
NLZ08_RS19040 (3842132) 3842132..3843376 - 1245 WP_000189541.1 esterase FrsA -
NLZ08_RS19045 (3843468) 3843468..3843926 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NLZ08_RS19050 (3844187) 3844187..3845644 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
NLZ08_RS19055 (3845701) 3845701..3846222 - 522 Protein_3737 peptide chain release factor H -
NLZ08_RS19060 (3846221) 3846221..3846424 - 204 Protein_3738 RtcB family protein -
NLZ08_RS19065 (3846670) 3846670..3847122 - 453 WP_097749298.1 GNAT family N-acetyltransferase -
NLZ08_RS19070 (3847132) 3847132..3847530 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NLZ08_RS19075 (3847533) 3847533..3847826 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NLZ08_RS19080 (3847878) 3847878..3848933 - 1056 WP_001226164.1 DNA polymerase IV -
NLZ08_RS19085 (3849004) 3849004..3849789 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
NLZ08_RS19090 (3849761) 3849761..3851473 + 1713 Protein_3744 flagellar biosynthesis protein FlhA -
NLZ08_RS19095 (3851689) 3851689..3852186 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T250793 WP_001263493.1 NZ_CP100530:c3847530-3847132 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT250793 WP_000554757.1 NZ_CP100530:c3847826-3847533 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References