Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3677152..3677846 Replicon chromosome
Accession NZ_CP100525
Organism Escherichia coli strain LH13-b

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag NLZ09_RS18035 Protein ID WP_001263493.1
Coordinates 3677152..3677550 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NLZ09_RS18040 Protein ID WP_000554757.1
Coordinates 3677553..3677846 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3672812) 3672812..3672892 - 81 NuclAT_11 - -
- (3672812) 3672812..3672892 - 81 NuclAT_11 - -
- (3672812) 3672812..3672892 - 81 NuclAT_11 - -
- (3672812) 3672812..3672892 - 81 NuclAT_11 - -
NLZ09_RS18005 (3672152) 3672152..3673396 - 1245 WP_000189541.1 esterase FrsA -
NLZ09_RS18010 (3673488) 3673488..3673946 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NLZ09_RS18015 (3674207) 3674207..3675664 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
NLZ09_RS18020 (3675721) 3675721..3676242 - 522 Protein_3527 peptide chain release factor H -
NLZ09_RS18025 (3676241) 3676241..3676444 - 204 Protein_3528 RtcB family protein -
NLZ09_RS18030 (3676690) 3676690..3677142 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
NLZ09_RS18035 (3677152) 3677152..3677550 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NLZ09_RS18040 (3677553) 3677553..3677846 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NLZ09_RS18045 (3677898) 3677898..3678953 - 1056 WP_001226164.1 DNA polymerase IV -
NLZ09_RS18050 (3679024) 3679024..3679809 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
NLZ09_RS18055 (3679781) 3679781..3681493 + 1713 Protein_3534 flagellar biosynthesis protein FlhA -
NLZ09_RS18060 (3681717) 3681717..3682214 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T250766 WP_001263493.1 NZ_CP100525:c3677550-3677152 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT250766 WP_000554757.1 NZ_CP100525:c3677846-3677553 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References