Detailed information of TA system TA025073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3406456..3407043
• Replicon: chromosome
• Accession: NC_014960
• Organism: Anaerolinea thermophila UNI-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: E8N2U2
• Locus tag: ANT_RS15290
• Protein ID: WP_013561433.1
• Coordinates: 3406762..3407043 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ANT_RS15285
• Protein ID: WP_013561432.1
• Coordinates: 3406456..3406752 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANT_RS15255	3401563..3401868	-	306	WP_013561426.1	hypothetical protein	-
ANT_RS16705	3401921..3402745	-	825	WP_013561427.1	CPBP family intramembrane metalloprotease	-
ANT_RS16710	3402897..3403301	-	405	WP_013561428.1	hypothetical protein	-
ANT_RS15270	3403484..3404581	-	1098	WP_013561429.1	phosphoserine transaminase	-
ANT_RS15275	3404848..3405663	-	816	WP_013561430.1	ATP-dependent sacrificial sulfur transferase LarE	-
ANT_RS15280	3405762..3406343	-	582	WP_013561431.1	HD domain-containing protein	-
ANT_RS15285	3406456..3406752	-	297	WP_013561432.1	HigA family addiction module antidote protein	Antitoxin
ANT_RS15290	3406762..3407043	-	282	WP_013561433.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANT_RS15295	3407269..3409134	+	1866	WP_013561434.1	molecular chaperone DnaK	-
ANT_RS15300	3409425..3410660	-	1236	WP_013561435.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
ANT_RS15305	3410741..3411505	+	765	WP_013561436.1	SDR family oxidoreductase	-
ANT_RS15310	3411556..3411729	-	174	WP_013561437.1	4Fe-4S binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10901.29 Da
• Isoelectric Point: 6.8703

>T25073 WP_013561433.1 NC_014960:c3407043-3406762 [Anaerolinea thermophila UNI-1]
VIKSFRDRSTESLFHGERHPSLPPNLQRIALRKLLILDAAADLDDLRVPPGNHLEKLRGDREGQYSIRINDRYRLCFVWQ
DGDAYEVEITNYH

Nucleotide

• Length: 282 bp

>T25073 NC_014960:c3407043-3406762 [Anaerolinea thermophila UNI-1]
GTGATTAAGTCCTTCCGCGATCGCTCTACCGAGAGCCTGTTTCACGGAGAACGGCATCCCTCTCTGCCGCCCAATCTGCA
AAGAATTGCCTTGCGCAAACTGCTCATCCTGGATGCCGCCGCCGATCTCGATGACCTGCGTGTTCCGCCCGGCAATCACC
TGGAGAAACTGCGCGGCGACCGCGAAGGACAGTACAGCATCCGTATCAACGACCGTTACCGTCTTTGCTTTGTCTGGCAG
GACGGAGACGCCTATGAAGTTGAAATTACCAATTACCACTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11138.88 Da
• Isoelectric Point: 5.1425

>AT25073 WP_013561432.1 NC_014960:c3406752-3406456 [Anaerolinea thermophila UNI-1]
MTREIPPIHPGEILLEEFLKPLGISQNALARALRVPPDRINAIVQGKRSITADTALRLARAFGTTPQFWLNLQARYDLDV
AQDRLEEQIEREVLPLIE

Nucleotide

• Length: 297 bp

>AT25073 NC_014960:c3406752-3406456 [Anaerolinea thermophila UNI-1]
ATGACCCGCGAAATTCCGCCTATCCATCCCGGTGAGATTTTACTGGAAGAGTTTCTCAAGCCGTTGGGCATCAGCCAGAA
CGCCCTGGCTCGCGCTCTGCGCGTTCCGCCCGACCGTATCAATGCCATTGTGCAGGGCAAGCGCAGTATCACGGCAGATA
CTGCTTTGCGTCTGGCGCGCGCTTTTGGCACAACGCCTCAGTTCTGGCTGAATTTGCAGGCGCGTTACGACCTGGATGTT
GCGCAGGATCGTCTGGAAGAGCAGATTGAGCGCGAGGTGCTTCCTTTGATTGAATAA

Structures

Toxin

• AlphaFold DB: E8N2U2