Detailed information of TA system TA025072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2387773..2388379
• Replicon: chromosome
• Accession: NC_014960
• Organism: Anaerolinea thermophila UNI-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E8MXT5
• Locus tag: ANT_RS10680
• Protein ID: WP_013560536.1
• Coordinates: 2387773..2388093 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ANT_RS10685
• Protein ID: WP_013560537.1
• Coordinates: 2388086..2388379 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANT_RS10640	2382982..2383416	-	435	WP_041454944.1	hypothetical protein	-
ANT_RS17385	2383490..2383630	-	141	WP_013560527.1	hypothetical protein	-
ANT_RS10645	2383706..2384158	-	453	WP_013560528.1	hypothetical protein	-
ANT_RS16475	2384160..2384573	-	414	WP_013560529.1	hypothetical protein	-
ANT_RS17390	2384591..2384755	-	165	WP_013560530.1	hypothetical protein	-
ANT_RS10655	2384975..2386123	+	1149	WP_172634617.1	ImmA/IrrE family metallo-endopeptidase	-
ANT_RS10660	2386120..2386629	+	510	WP_013560532.1	DUF4411 family protein	-
ANT_RS10665	2386798..2387034	+	237	WP_013560533.1	DUF4160 domain-containing protein	-
ANT_RS10670	2387015..2387314	+	300	WP_013560534.1	DUF2442 domain-containing protein	-
ANT_RS16480	2387283..2387687	-	405	WP_162787686.1	A24 family peptidase	-
ANT_RS10680	2387773..2388093	+	321	WP_013560536.1	hypothetical protein	Toxin
ANT_RS10685	2388086..2388379	+	294	WP_013560537.1	helix-turn-helix domain-containing protein	Antitoxin
ANT_RS16485	2388384..2389256	-	873	WP_013560538.1	N-acetylmuramoyl-L-alanine amidase	-
ANT_RS10695	2389246..2390196	-	951	WP_172634618.1	CAP domain-containing protein	-
ANT_RS10700	2390511..2391377	-	867	WP_013560540.1	hypothetical protein	-
ANT_RS10705	2391476..2392399	-	924	WP_013560541.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2375439..2424305	48866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12552.41 Da
• Isoelectric Point: 8.5130

>T25072 WP_013560536.1 NC_014960:2387773-2388093 [Anaerolinea thermophila UNI-1]
MHFIETSVFTRRILELMSDDEYRALQIFLSTRPDAGDIIPGSGGLRKVRWHTGTTGKRGGIRLIYYWVVRDDTILLLFAF
RKNERSDLTPEQIRILRRIVEEEYHE

Nucleotide

• Length: 321 bp

>T25072 NC_014960:2387773-2388093 [Anaerolinea thermophila UNI-1]
ATGCACTTCATCGAGACTTCCGTCTTCACCCGTCGAATTCTGGAATTAATGAGCGATGATGAATATCGAGCCCTGCAAAT
CTTTCTGAGCACCAGACCAGATGCAGGAGACATTATTCCGGGAAGCGGTGGACTTCGTAAAGTACGATGGCATACTGGTA
CCACAGGTAAACGTGGCGGCATTCGGCTGATTTATTACTGGGTGGTTCGTGACGATACCATACTGCTTTTGTTCGCTTTC
CGAAAAAATGAACGTTCCGATCTAACCCCAGAACAGATTCGAATCCTGCGCCGAATTGTCGAGGAGGAATACCATGAATA
A

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11014.68 Da
• Isoelectric Point: 11.0713

>AT25072 WP_013560537.1 NC_014960:2388086-2388379 [Anaerolinea thermophila UNI-1]
MNNETFTELLSSVQEGADILRGRRPPSRVFDINFQDVKAIRERLGLSQRQFAALMGVSIDTLQNWEQNRRKPRGAARTLL
LIAAKHPETILDILKTA

Nucleotide

• Length: 294 bp

>AT25072 NC_014960:2388086-2388379 [Anaerolinea thermophila UNI-1]
ATGAATAACGAAACTTTCACTGAATTACTCTCCAGCGTGCAGGAAGGGGCTGACATTCTTCGCGGACGACGCCCTCCTTC
TCGCGTTTTTGACATTAACTTCCAGGACGTCAAGGCGATCCGTGAGCGCCTGGGGCTTTCACAGCGCCAATTTGCCGCAT
TGATGGGCGTGAGCATTGACACCCTGCAAAACTGGGAACAAAACCGCCGTAAACCCCGTGGAGCAGCCAGAACACTGCTT
CTCATTGCCGCCAAACATCCCGAAACCATTCTGGACATCCTGAAAACTGCCTGA

Structures

Toxin

• AlphaFold DB: E8MXT5