Detailed information of TA system TA025070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1060292..1060806
• Replicon: chromosome
• Accession: NC_014935
• Organism: Nitratifractor salsuginis DSM 16511

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6WXQ4
• Locus tag: NITSA_RS05365
• Protein ID: WP_013554004.1
• Coordinates: 1060543..1060806 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E6WXQ3
• Locus tag: NITSA_RS05360
• Protein ID: WP_013554003.1
• Coordinates: 1060292..1060546 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NITSA_RS05345	1056413..1057360	+	948	WP_042204239.1	type I-B CRISPR-associated protein Cas7/Csh2	-
NITSA_RS05350	1057357..1058076	+	720	WP_013554001.1	CRISPR-associated protein Cas5	-
NITSA_RS05355	1058054..1060237	+	2184	WP_148224944.1	CRISPR-associated helicase Cas3'	-
NITSA_RS05360	1060292..1060546	+	255	WP_013554003.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
NITSA_RS05365	1060543..1060806	+	264	WP_013554004.1	Txe/YoeB family addiction module toxin	Toxin
NITSA_RS05370	1060799..1061296	+	498	WP_013554005.1	Dna2/Cas4 domain-containing protein	-
NITSA_RS05375	1061305..1061583	+	279	WP_013554006.1	CRISPR-associated endonuclease Cas2	-
NITSA_RS05380	1061592..1062590	+	999	WP_013554007.1	CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1053440..1067979	14539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10342.95 Da
• Isoelectric Point: 9.6578

>T25070 WP_013554004.1 NC_014935:1060543-1060806 [Nitratifractor salsuginis DSM 16511]
MIVAFSDIAWDDYLYWQQTNKKVLKRINTLIKDIKRNPNSAEGIGKPEMLRGNLSGYFSRRITDEHRLVYKIDGDYMIIA
QCRFHYV

Nucleotide

• Length: 264 bp

>T25070 NC_014935:1060543-1060806 [Nitratifractor salsuginis DSM 16511]
ATGATTGTCGCCTTTTCGGATATAGCCTGGGATGACTATCTCTACTGGCAACAGACCAATAAAAAGGTGCTCAAACGCAT
CAATACGCTCATCAAAGATATCAAGCGCAATCCTAATAGTGCGGAGGGTATCGGAAAACCGGAGATGCTCAGAGGAAATC
TGAGCGGTTATTTTTCCAGACGAATCACTGATGAACATCGGCTTGTCTATAAGATAGACGGTGACTACATGATTATCGCG
CAGTGTAGATTTCACTATGTTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9812.03 Da
• Isoelectric Point: 4.5052

>AT25070 WP_013554003.1 NC_014935:1060292-1060546 [Nitratifractor salsuginis DSM 16511]
MRAVNYSHARNNLKSLIDDVDQNFEEYLITTKNNTKAMLISVDEYNAMKETLYLLSTPANRERLLESIQQIEAGRFSEKE
LIEE

Nucleotide

• Length: 255 bp

>AT25070 NC_014935:1060292-1060546 [Nitratifractor salsuginis DSM 16511]
ATGCGAGCAGTCAACTATTCACATGCCAGAAACAATCTCAAATCGCTTATAGATGATGTCGATCAGAACTTTGAAGAGTA
TCTGATCACCACCAAAAACAATACCAAAGCGATGCTCATCTCTGTCGATGAGTACAATGCCATGAAAGAGACGCTCTATC
TGCTCTCTACCCCCGCAAACAGGGAACGGCTGCTGGAATCGATTCAGCAGATTGAAGCGGGCAGGTTTAGTGAAAAAGAG
TTGATTGAAGAATGA

Structures

Toxin

• AlphaFold DB: E6WXQ4

Antitoxin

• AlphaFold DB: E6WXQ3