Detailed information of TA system TA025065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2439955..2440478
• Replicon: chromosome
• Accession: NC_014925
• Organism: Staphylococcus pseudintermedius HKU10-03

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: SPSINT_RS11665
• Protein ID: WP_015729746.1
• Coordinates: 2439955..2440221 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2P5PI69
• Locus tag: SPSINT_RS11670
• Protein ID: WP_015729747.1
• Coordinates: 2440221..2440478 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPSINT_RS11660	2437986..2439479	+	1494	WP_014612817.1	malate dehydrogenase (quinone)	-
SPSINT_RS12705	2439493..2439594	+	102	WP_019169745.1	DUF2648 domain-containing protein	-
SPSINT_RS11665	2439955..2440221	-	267	WP_015729746.1	Txe/YoeB family addiction module toxin	Toxin
SPSINT_RS11670	2440221..2440478	-	258	WP_015729747.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPSINT_RS11675	2440672..2442576	-	1905	WP_015729748.1	triacylglycerol lipase	-
SPSINT_RS11680	2442836..2443330	+	495	WP_015729749.1	membrane protein	-
SPSINT_RS11685	2443624..2444430	-	807	WP_015729750.1	arylamine N-acetyltransferase	-
SPSINT_RS11690	2444960..2445142	+	183	WP_041613203.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10541.08 Da
• Isoelectric Point: 10.1602

>T25065 WP_015729746.1 NC_014925:c2440221-2439955 [Staphylococcus pseudintermedius HKU10-03]
MGNYSVLIKNSAKNDLKKIKKSLLKEQFSKIIEVLKVNPYEASQSFEKLQPKHSARYSRRLNHQHRVVYTVDDEKREVYI
FSAWSYYE

Nucleotide

• Length: 267 bp

>T25065 NC_014925:c2440221-2439955 [Staphylococcus pseudintermedius HKU10-03]
ATGGGTAATTATTCTGTCTTAATTAAGAATTCAGCCAAAAATGACTTGAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGGTTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCATATTATGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9836.85 Da
• Isoelectric Point: 4.1100

>AT25065 WP_015729747.1 NC_014925:c2440478-2440221 [Staphylococcus pseudintermedius HKU10-03]
MIIKTPTNARKEFYELLKQVNDNHEPIIISGSKSENNAVIVSQEDWDGIQETLFLEYTGTMDKVRAREKDDSGYTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT25065 NC_014925:c2440478-2440221 [Staphylococcus pseudintermedius HKU10-03]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATGGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2P5PI69