Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1279235..1279962 Replicon chromosome
Accession NZ_CP100486
Organism Escherichia coli strain LH85-a

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NLZ16_RS06130 Protein ID WP_000547555.1
Coordinates 1279235..1279546 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NLZ16_RS06135 Protein ID WP_000126294.1
Coordinates 1279543..1279962 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ16_RS06105 (1275170) 1275170..1276879 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NLZ16_RS06110 (1276889) 1276889..1277431 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NLZ16_RS06115 (1277431) 1277431..1278198 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NLZ16_RS06120 (1278195) 1278195..1278605 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NLZ16_RS06125 (1278598) 1278598..1279068 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NLZ16_RS06130 (1279235) 1279235..1279546 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NLZ16_RS06135 (1279543) 1279543..1279962 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NLZ16_RS06140 (1280041) 1280041..1281465 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NLZ16_RS06145 (1281474) 1281474..1282931 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NLZ16_RS06150 (1283191) 1283191..1284201 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NLZ16_RS06155 (1284350) 1284350..1284877 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T250615 WP_000547555.1 NZ_CP100486:1279235-1279546 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT250615 WP_000126294.1 NZ_CP100486:1279543-1279962 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References