Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 664861..665660 Replicon chromosome
Accession NZ_CP100486
Organism Escherichia coli strain LH85-a

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NLZ16_RS03205 Protein ID WP_000347270.1
Coordinates 664861..665325 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NLZ16_RS03210 Protein ID WP_001551693.1
Coordinates 665325..665660 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ16_RS03175 (659862) 659862..660296 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NLZ16_RS03180 (660314) 660314..661192 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NLZ16_RS03185 (661182) 661182..661961 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NLZ16_RS03190 (661972) 661972..662445 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NLZ16_RS03195 (662468) 662468..663748 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NLZ16_RS03200 (663997) 663997..664806 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NLZ16_RS03205 (664861) 664861..665325 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NLZ16_RS03210 (665325) 665325..665660 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NLZ16_RS03215 (665809) 665809..667380 - 1572 WP_001273738.1 galactarate dehydratase -
NLZ16_RS03220 (667755) 667755..669089 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NLZ16_RS03225 (669105) 669105..669875 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T250610 WP_000347270.1 NZ_CP100486:c665325-664861 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT250610 WP_001551693.1 NZ_CP100486:c665660-665325 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References