Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4273047..4273569 Replicon chromosome
Accession NC_014923
Organism Mesorhizobium ciceri biovar biserrulae WSM1271

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MESCI_RS20760 Protein ID WP_164920056.1
Coordinates 4273384..4273569 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MESCI_RS20755 Protein ID WP_013531910.1
Coordinates 4273047..4273346 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MESCI_RS20740 4269771..4270058 - 288 WP_013531907.1 succinate dehydrogenase assembly factor 2 -
MESCI_RS20745 4270151..4272259 + 2109 WP_013531908.1 ATP-dependent DNA helicase RecG -
MESCI_RS20750 4272211..4272966 - 756 WP_013531909.1 DUF502 domain-containing protein -
MESCI_RS20755 4273047..4273346 - 300 WP_013531910.1 HigA family addiction module antidote protein Antitoxin
MESCI_RS20760 4273384..4273569 - 186 WP_164920056.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MESCI_RS20765 4273580..4275403 - 1824 WP_013531911.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
MESCI_RS20770 4275576..4276931 - 1356 WP_013531912.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
MESCI_RS20775 4277079..4277516 - 438 WP_013531913.1 hypothetical protein -
MESCI_RS20780 4277599..4278345 - 747 WP_013531914.1 cytochrome c biogenesis CcdA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-61)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-68)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6923.75 Da        Isoelectric Point: 6.7112

>T25061 WP_164920056.1 NC_014923:c4273569-4273384 [Mesorhizobium ciceri biovar biserrulae WSM1271]
ILRAIANAAQLNDLRSPPGNHLEALNWSRAGQHSIRINDQWRICFKWTAAGAEDVEIVDYH

Download         Length: 186 bp

>T25061 NC_014923:c4273569-4273384 [Mesorhizobium ciceri biovar biserrulae WSM1271]
ATCTTGCGCGCAATTGCCAATGCTGCCCAACTGAATGACCTTCGATCGCCGCCCGGAAATCATCTTGAAGCCCTGAACTG
GAGCCGAGCCGGACAGCATTCAATTCGCATCAACGACCAGTGGCGCATCTGCTTCAAATGGACCGCTGCTGGCGCTGAAG
ACGTGGAGATCGTCGATTACCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11370.24 Da        Isoelectric Point: 5.9082

>AT25061 WP_013531910.1 NC_014923:c4273346-4273047 [Mesorhizobium ciceri biovar biserrulae WSM1271]
MLKFARPVHPGEFLREEYLVPLGMSAGALAKKLNLPRTRIERIAKEEIGLTPDTALRLARFFNTTPEFWMNFQQAYELET
EARKLAPELEKIEPLEVAA

Download         Length: 300 bp

>AT25061 NC_014923:c4273346-4273047 [Mesorhizobium ciceri biovar biserrulae WSM1271]
ATGCTCAAGTTCGCACGTCCAGTTCATCCTGGAGAATTCCTGCGTGAAGAATATCTCGTCCCGCTCGGCATGAGTGCAGG
CGCACTGGCGAAAAAGCTCAACCTTCCGCGCACCCGTATCGAACGCATTGCCAAGGAAGAGATCGGCCTGACTCCCGATA
CCGCTCTTCGCCTCGCCAGATTTTTCAACACCACGCCCGAATTCTGGATGAATTTTCAACAGGCCTATGAGCTTGAAACC
GAAGCCAGAAAGCTGGCGCCGGAGCTCGAGAAGATTGAGCCGTTGGAAGTAGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References