Detailed information of TA system TA025057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1325388..1326027
• Replicon: chromosome
• Accession: NC_014922
• Organism: Haemophilus influenzae F3047

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: HICON_RS06520
• Protein ID: WP_005650215.1
• Coordinates: 1325388..1325693 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HICON_RS06525
• Protein ID: WP_005650217.1
• Coordinates: 1325704..1326027 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HICON_RS06510	1323466..1324440	-	975	WP_006995878.1	zinc transporter permease subunit ZevB	-
HICON_RS06515	1324443..1325063	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
HICON_RS06520	1325388..1325693	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HICON_RS06525	1325704..1326027	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
HICON_RS06530	1326190..1327860	+	1671	WP_005663485.1	energy-dependent translational throttle protein EttA	-
HICON_RS06535	1327921..1328262	+	342	WP_006995879.1	SirB2 family protein	-
HICON_RS06540	1328267..1330237	+	1971	WP_013527594.1	tRNA(Met) cytidine acetyltransferase TmcA	-
HICON_RS10505	1330234..1330407	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T25057 WP_005650215.1 NC_014922:1325388-1325693 [Haemophilus influenzae F3047]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T25057 NC_014922:1325388-1325693 [Haemophilus influenzae F3047]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT25057 WP_005650217.1 NC_014922:1325704-1326027 [Haemophilus influenzae F3047]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT25057 NC_014922:1325704-1326027 [Haemophilus influenzae F3047]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70