Detailed information of TA system TA025049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 617568..618211
• Replicon: chromosome
• Accession: NC_014920
• Organism: Haemophilus influenzae F3031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C2R1D6
• Locus tag: HIBPF_RS03135
• Protein ID: WP_041174909.1
• Coordinates: 617873..618211 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIBPF_RS03130
• Protein ID: WP_006995911.1
• Coordinates: 617568..617876 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIBPF_RS03095	612792..613904	+	1113	WP_013525746.1	iron-sulfur cluster carrier protein ApbC	-
HIBPF_RS03100	614135..614398	+	264	WP_171034549.1	antitoxin MazE	-
HIBPF_RS03105	614398..614751	+	354	WP_006995906.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HIBPF_RS03110	614864..615037	-	174	Protein_601	antitoxin	-
HIBPF_RS03115	615049..615711	-	663	WP_013525747.1	NAD(P)H-dependent oxidoreductase	-
HIBPF_RS03120	615825..616499	+	675	WP_013525748.1	N-acylneuraminate cytidylyltransferase	-
HIBPF_RS10015	616579..617154	+	576	Protein_604	DDE-type integrase/transposase/recombinase	-
HIBPF_RS03130	617568..617876	-	309	WP_006995911.1	helix-turn-helix domain-containing protein	Antitoxin
HIBPF_RS03135	617873..618211	-	339	WP_041174909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIBPF_RS03140	618844..621036	-	2193	WP_013525749.1	DUF927 domain-containing protein	-
HIBPF_RS03145	621024..621416	-	393	WP_006995914.1	hypothetical protein	-
HIBPF_RS03150	621409..622092	-	684	WP_013525750.1	hypothetical protein	-
HIBPF_RS03155	622079..622282	-	204	WP_006995916.1	hypothetical protein	-
HIBPF_RS03160	622272..622643	-	372	WP_013525751.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-80)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13150.10 Da
• Isoelectric Point: 9.2488

>T25049 WP_041174909.1 NC_014920:c618211-617873 [Haemophilus influenzae F3031]
VKLSFIELPPFERYRKAHLSDDEYRAFQNELLENLEKGDVIQNAGGLRKIRIADSERNKGKRGGARVIYYYLIRKSQILL
VTAYSKNRCEDLTTEQYKILAKLVKEIEELEQ

Nucleotide

• Length: 339 bp

>T25049 NC_014920:c618211-617873 [Haemophilus influenzae F3031]
GTGAAATTATCATTTATTGAATTACCGCCCTTTGAGCGTTACAGAAAAGCACATTTATCAGATGATGAATATAGGGCTTT
CCAGAATGAGCTATTAGAAAACCTTGAAAAGGGAGACGTGATACAAAATGCTGGTGGCTTGCGGAAAATTAGGATTGCCG
ATAGTGAAAGAAATAAAGGGAAACGAGGTGGCGCACGAGTGATTTATTACTATCTCATACGTAAATCGCAGATATTACTC
GTTACCGCTTACAGTAAAAACAGATGCGAAGATTTAACCACAGAGCAATATAAAATTTTAGCTAAACTGGTGAAAGAGAT
AGAGGAGCTAGAGCAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11550.34 Da
• Isoelectric Point: 9.9619

>AT25049 WP_006995911.1 NC_014920:c617876-617568 [Haemophilus influenzae F3031]
MSKLFDELIEGLNAYKDFTQGKITLRVETRERIEPASITAEEVKAIREKLNLSQAVFAQKLRTSVRTYQGWEQGKTKPSA
HASLLLRMVDKAPQTFELIAGI

Nucleotide

• Length: 309 bp

>AT25049 NC_014920:c617876-617568 [Haemophilus influenzae F3031]
ATGAGTAAATTATTTGATGAGCTTATAGAGGGGCTAAATGCCTATAAAGACTTTACACAGGGGAAAATAACGCTCAGAGT
AGAGACGCGTGAACGAATTGAACCTGCAAGCATTACAGCAGAGGAAGTGAAAGCAATTAGAGAGAAATTAAACCTTTCTC
AAGCCGTTTTTGCGCAGAAGTTACGAACTAGTGTAAGAACCTATCAAGGTTGGGAGCAAGGGAAAACCAAACCTAGCGCA
CACGCCTCCTTATTGTTGCGCATGGTGGATAAAGCCCCGCAAACCTTTGAGCTCATCGCAGGGATATAA

Structures

Toxin

• AlphaFold DB: A0A5C2R1D6