Detailed information of TA system TA025037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 499322..499927
• Replicon: chromosome
• Accession: NC_014844
• Organism: Desulfovibrio aespoeensis Aspo-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E6VXM7
• Locus tag: DAES_RS02280
• Protein ID: WP_013513422.1
• Coordinates: 499649..499927 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: DAES_RS02275
• Protein ID: WP_013513421.1
• Coordinates: 499322..499636 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAES_RS02230	494923..496041	+	1119	WP_013513413.1	DUF2586 domain-containing protein	-
DAES_RS02235	496053..496505	+	453	WP_013513414.1	DUF2597 family protein	-
DAES_RS02240	496515..496715	+	201	WP_013513415.1	TraR/DksA C4-type zinc finger protein	-
DAES_RS17775	496715..496936	+	222	WP_173358446.1	hypothetical protein	-
DAES_RS02245	496863..497450	+	588	WP_049776366.1	transglycosylase SLT domain-containing protein	-
DAES_RS16820	497462..497869	+	408	WP_157864788.1	hypothetical protein	-
DAES_RS02255	498077..498277	+	201	WP_041271318.1	hypothetical protein	-
DAES_RS02260	498298..498579	+	282	WP_013513418.1	putative phage tail assembly chaperone	-
DAES_RS17780	498600..498743	+	144	WP_013513419.1	hypothetical protein	-
DAES_RS02270	498800..499027	-	228	WP_013513420.1	hypothetical protein	-
DAES_RS02275	499322..499636	-	315	WP_013513421.1	HigA family addiction module antidote protein	Antitoxin
DAES_RS02280	499649..499927	-	279	WP_013513422.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DAES_RS02285	499978..501783	+	1806	WP_013513423.1	phage tail tape measure protein	-
DAES_RS02290	501798..502130	+	333	WP_013513424.1	DUF2590 family protein	-
DAES_RS02295	502127..503302	+	1176	WP_013513425.1	baseplate J/gp47 family protein	-
DAES_RS17200	503375..503593	-	219	WP_013513426.1	hypothetical protein	-
DAES_RS02305	503772..504386	+	615	WP_013513427.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	463353..526952	63599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10739.28 Da
• Isoelectric Point: 9.4032

>T25037 WP_013513422.1 NC_014844:c499927-499649 [Pseudodesulfovibrio aespoeensis Aspo-2]
MIKSFKHKGLEKFFRTGTTKGIQAKHADKLERILDFLHAADEAQEMNLPGFNLHPLIGTLKDHWSVKVSGNWRVTFYFED
GRAYVVNYLDYH

Nucleotide

• Length: 279 bp

>T25037 NC_014844:c499927-499649 [Pseudodesulfovibrio aespoeensis Aspo-2]
ATGATCAAGAGCTTCAAGCACAAAGGGCTGGAAAAGTTTTTCAGGACCGGCACCACCAAGGGCATCCAGGCGAAGCATGC
CGACAAGCTGGAGCGAATTCTTGATTTCCTGCATGCCGCCGATGAGGCCCAGGAAATGAACCTCCCCGGTTTCAACCTCC
ACCCGCTGATCGGCACGCTGAAAGATCATTGGTCCGTGAAGGTCTCCGGCAACTGGCGGGTGACTTTTTACTTTGAAGAC
GGCCGTGCGTATGTCGTCAACTACCTTGACTACCACTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11604.44 Da
• Isoelectric Point: 10.3854

>AT25037 WP_013513421.1 NC_014844:c499636-499322 [Pseudodesulfovibrio aespoeensis Aspo-2]
MIERTRKPVHPGGIILRECIEPLNLSVKAFAEQLGVSRKTLSKIVNERGAVTPDMALRLSRRLGTTPQIWMNLQGRHDLW
VAEHETDGWKAAVPKDKATPSPDA

Nucleotide

• Length: 315 bp

>AT25037 NC_014844:c499636-499322 [Pseudodesulfovibrio aespoeensis Aspo-2]
ATGATTGAGAGAACGAGAAAGCCCGTCCATCCCGGAGGCATTATCCTGCGGGAGTGCATCGAGCCGCTGAACCTGTCGGT
CAAGGCCTTTGCCGAGCAGCTGGGCGTCAGCCGTAAGACCCTGTCCAAGATCGTCAACGAGCGGGGCGCGGTCACGCCGG
ACATGGCCCTGCGCCTCTCCCGCCGCCTGGGCACCACGCCGCAGATCTGGATGAACTTGCAGGGCAGGCACGACCTCTGG
GTGGCCGAGCACGAGACGGACGGCTGGAAAGCCGCCGTGCCCAAGGACAAGGCCACACCCTCGCCGGACGCGTAG

Structures

Toxin

• AlphaFold DB: E6VXM7