Detailed information of TA system TA025036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 336148..336653
• Replicon: plasmid pPAT9B02
• Accession: NC_014839
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6WLQ0
• Locus tag: PAT9B_RS30105
• Protein ID: WP_013512164.1
• Coordinates: 336148..336411 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E6WLQ1
• Locus tag: PAT9B_RS25480
• Protein ID: WP_013512165.1
• Coordinates: 336408..336653 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS25460	331997..332911	-	915	WP_013512160.1	ABC transporter permease	-
PAT9B_RS25465	332913..333761	-	849	WP_013512161.1	ABC transporter permease	-
PAT9B_RS25470	333758..335251	-	1494	WP_013512162.1	ABC transporter substrate-binding protein	-
PAT9B_RS25475	335418..336074	+	657	WP_013512163.1	GntR family transcriptional regulator	-
PAT9B_RS30105	336148..336411	-	264	WP_013512164.1	Txe/YoeB family addiction module toxin	Toxin
PAT9B_RS25480	336408..336653	-	246	WP_013512165.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PAT9B_RS25485	336726..338027	-	1302	WP_150105872.1	glycoside hydrolase family 10 protein	-
PAT9B_RS25490	338235..339038	+	804	WP_013512167.1	arylamine N-acetyltransferase	-
PAT9B_RS25495	339053..339361	+	309	WP_013512168.1	hypothetical protein	-
PAT9B_RS25500	339358..340017	-	660	WP_013512169.1	DUF1345 domain-containing protein	-
PAT9B_RS25505	340087..340278	+	192	Protein_310	TonB-dependent receptor	-
PAT9B_RS25510	340334..341363	+	1030	Protein_311	hemin-degrading factor	-
PAT9B_RS30760	341390..341521	+	132	Protein_312	hemin ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	wza / manC / manB / shuS	1..394054	394054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10517.08 Da
• Isoelectric Point: 10.1238

>T25036 WP_013512164.1 NC_014839:c336411-336148 [Pantoea sp. At-9b]
MKTLFTKRGAKDYNYWKENDPKLFERLRELIKATHENPLQGIGKPEQLRHYKNPALYSRRISREHRLVYSVNNGEITIVA
CRFYYEL

Nucleotide

• Length: 264 bp

>T25036 NC_014839:c336411-336148 [Pantoea sp. At-9b]
TTGAAAACGTTATTTACCAAACGTGGTGCCAAAGATTACAACTACTGGAAAGAGAATGATCCAAAGCTGTTTGAACGTCT
CAGGGAGCTGATTAAAGCTACTCATGAGAATCCGTTGCAAGGTATTGGCAAACCTGAACAGTTAAGGCATTACAAGAATC
CCGCCCTTTATTCACGGCGTATAAGCCGGGAGCATCGACTTGTTTACAGCGTAAATAATGGTGAAATCACGATAGTGGCT
TGTCGATTCTATTATGAACTTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9102.16 Da
• Isoelectric Point: 4.0548

>AT25036 WP_013512165.1 NC_014839:c336653-336408 [Pantoea sp. At-9b]
MHTLTFTDARKHFADTMQRVSDNAEPVRILRRDAPDVVMIDAAEYDAMIETLYLFSNPANAAYLNASLNELDNGDVVEID
V

Nucleotide

• Length: 246 bp

>AT25036 NC_014839:c336653-336408 [Pantoea sp. At-9b]
ATGCATACACTAACATTTACCGATGCCCGTAAACATTTCGCAGACACCATGCAGCGCGTGTCTGACAATGCCGAACCTGT
ACGCATTTTACGCCGTGACGCGCCGGACGTGGTCATGATTGATGCAGCGGAATATGACGCAATGATAGAAACGTTGTATC
TGTTCAGCAATCCGGCCAATGCGGCGTATTTGAACGCCTCGCTAAATGAGTTGGACAACGGCGACGTGGTTGAAATCGAC
GTTTGA

Structures

Toxin

• AlphaFold DB: E6WLQ0

Antitoxin

• AlphaFold DB: E6WLQ1