Detailed information of TA system TA025035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 534036..534538
• Replicon: plasmid pPAT9B01
• Accession: NC_014838
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6WIZ9
• Locus tag: PAT9B_RS22860
• Protein ID: WP_013511658.1
• Coordinates: 534284..534538 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E6WIZ8
• Locus tag: PAT9B_RS22855
• Protein ID: WP_013511657.1
• Coordinates: 534036..534287 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS22835	529474..530367	-	894	WP_013511653.1	AraC family transcriptional regulator	-
PAT9B_RS22840	530477..531469	+	993	WP_013511654.1	class I SAM-dependent methyltransferase	-
PAT9B_RS22845	531459..531812	-	354	WP_013511655.1	MmcQ/YjbR family DNA-binding protein	-
PAT9B_RS22850	531879..533819	-	1941	WP_013511656.1	methyl-accepting chemotaxis protein	-
PAT9B_RS22855	534036..534287	+	252	WP_013511657.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
PAT9B_RS22860	534284..534538	+	255	WP_013511658.1	Txe/YoeB family addiction module toxin	Toxin
PAT9B_RS22865	534574..535041	-	468	WP_013511659.1	heme-binding protein	-
PAT9B_RS22870	535087..536481	-	1395	WP_013511660.1	cytochrome c	-
PAT9B_RS22875	536485..538077	-	1593	WP_013511661.1	GMC family oxidoreductase	-
PAT9B_RS22880	538102..538758	-	657	WP_013511662.1	sorbitol dehydrogenase family protein	-
PAT9B_RS22885	538774..539250	-	477	WP_013511663.1	heme-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..793953	793953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9996.45 Da
• Isoelectric Point: 9.3796

>T25035 WP_013511658.1 NC_014838:534284-534538 [Pantoea sp. At-9b]
VKLTWSQEAWDDYLYWQDTDKRMVKKINELIKDAKRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAVSDGALLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T25035 NC_014838:534284-534538 [Pantoea sp. At-9b]
GTGAAATTAACATGGTCGCAGGAAGCGTGGGATGACTATCTCTACTGGCAGGATACAGACAAAAGAATGGTCAAAAAAAT
CAATGAACTCATCAAAGATGCCAAAAGAACGCCTTTTGAAGGGAAAGGTAAACCCGAGCCCTTAAAGCATAATCTGGCCG
GATTCTGGTCACGAAGAATCACAGAAGAACATCGTTTGGTTTATGCAGTCAGCGATGGCGCACTGTTAATCGCCGCCTGC
CGTTATCACTACTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9579.80 Da
• Isoelectric Point: 4.5052

>AT25035 WP_013511657.1 NC_014838:534036-534287 [Pantoea sp. At-9b]
MRTITYSEARQHLSETMMKTVEDRAPILITRQNGDDCVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLRNGNTIEKE
IDE

Nucleotide

• Length: 252 bp

>AT25035 NC_014838:534036-534287 [Pantoea sp. At-9b]
ATGCGCACCATAACTTACAGTGAAGCACGACAGCATCTTTCGGAAACGATGATGAAAACGGTAGAGGATCGAGCCCCCAT
TTTGATCACCCGACAGAATGGTGACGACTGTGTATTGATGTCGTTGGAAGAATACAATTCATTGGAAGAGACCGCCTATC
TTCTGCGTTCACCCGCCAATGCCAAACGATTGATGAACTCAATTGAAAGCTTACGTAATGGGAATACCATTGAAAAGGAA
ATTGATGAGTGA

Structures

Toxin

• AlphaFold DB: E6WIZ9

Antitoxin

• AlphaFold DB: E6WIZ8