Detailed information of TA system TA025033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 266868..267522
• Replicon: plasmid pPAT9B01
• Accession: NC_014838
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PAT9B_RS21595
• Protein ID: WP_041526096.1
• Coordinates: 267151..267522 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: E6WKG2
• Locus tag: PAT9B_RS21590
• Protein ID: WP_013511403.1
• Coordinates: 266868..267167 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS21560	262549..262812	-	264	WP_013511397.1	hypothetical protein	-
PAT9B_RS21565	262946..263185	+	240	WP_013511398.1	hypothetical protein	-
PAT9B_RS21570	263187..263510	+	324	WP_013511399.1	hypothetical protein	-
PAT9B_RS21575	263767..264822	+	1056	WP_013511400.1	hypothetical protein	-
PAT9B_RS21580	264803..265252	+	450	WP_013511401.1	hypothetical protein	-
PAT9B_RS29425	265231..266031	+	801	WP_013511402.1	hypothetical protein	-
PAT9B_RS21590	266868..267167	-	300	WP_013511403.1	helix-turn-helix domain-containing protein	Antitoxin
PAT9B_RS21595	267151..267522	-	372	WP_041526096.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAT9B_RS21600	267611..268552	-	942	WP_013511405.1	LysR family transcriptional regulator	-
PAT9B_RS21605	268688..269689	+	1002	WP_013511406.1	zinc-binding alcohol dehydrogenase family protein	-
PAT9B_RS21610	269776..270429	+	654	WP_013511407.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..793953	793953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14440.97 Da
• Isoelectric Point: 10.1376

>T25033 WP_041526096.1 NC_014838:c267522-267151 [Pantoea sp. At-9b]
IWKVEATDLFWDWLQGQDEPLRLDMLAALRLLAEEGPLLGRPFVDTLAFSVLPNMKELRVQSKGRPIRAFFVFDPLRHAI
ILCAGNKQGKDQKRFYKKMLHITAQQYHKHLNLIEKKCNENIR

Nucleotide

• Length: 372 bp

>T25033 NC_014838:c267522-267151 [Pantoea sp. At-9b]
ATATGGAAAGTTGAAGCAACCGATCTGTTTTGGGATTGGCTGCAAGGGCAGGATGAACCGTTGCGATTAGATATGTTGGC
TGCGTTGCGGTTGCTGGCTGAGGAAGGGCCACTTTTGGGCCGCCCTTTTGTCGATACGCTGGCTTTCAGTGTTTTGCCCA
ATATGAAGGAGCTAAGGGTACAGAGTAAGGGGCGTCCAATTCGCGCTTTCTTTGTCTTTGACCCATTACGCCACGCCATT
ATCTTGTGTGCCGGGAATAAACAAGGCAAAGATCAGAAACGCTTCTACAAAAAAATGCTGCACATCACCGCACAGCAATA
TCATAAGCATTTGAACTTAATAGAGAAGAAATGCAATGAAAACATTAGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11138.90 Da
• Isoelectric Point: 7.3189

>AT25033 WP_013511403.1 NC_014838:c267167-266868 [Pantoea sp. At-9b]
MKTLDEMLATLTPEQLQQVTARHAQMRLENQLYQLREGLHRTQRQQADAMAIAQSSVAAIESRGVELKISTLKRYIEALG
GELLLGVKMPDGRHVELTL

Nucleotide

• Length: 300 bp

>AT25033 NC_014838:c267167-266868 [Pantoea sp. At-9b]
ATGAAAACATTAGATGAAATGTTGGCAACATTAACACCCGAACAATTGCAGCAAGTCACCGCACGCCATGCACAAATGCG
ACTTGAAAATCAATTGTACCAGCTGCGTGAAGGTTTACACCGAACTCAGCGTCAGCAAGCGGACGCCATGGCCATCGCGC
AATCATCAGTGGCAGCGATTGAGTCACGGGGGGTTGAGTTAAAAATTTCCACCCTAAAGCGCTACATTGAGGCATTGGGC
GGCGAGTTGTTGTTGGGGGTTAAAATGCCCGATGGCCGACATGTTGAGTTGACCCTATAG

Structures

Antitoxin

• AlphaFold DB: E6WKG2