Detailed information of TA system TA025031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4128397..4129072
• Replicon: chromosome
• Accession: NC_014837
• Organism: Pantoea sp. At-9b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E6WH88
• Locus tag: PAT9B_RS19085
• Protein ID: WP_013510910.1
• Coordinates: 4128764..4129072 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PAT9B_RS19080
• Protein ID: WP_013510909.1
• Coordinates: 4128397..4128726 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAT9B_RS19070	4125234..4126913	-	1680	WP_013510907.1	potassium-transporting ATPase subunit KdpA	-
PAT9B_RS29825	4126913..4127002	-	90	WP_071783594.1	K(+)-transporting ATPase subunit F	-
PAT9B_RS19075	4127111..4128277	-	1167	WP_013510908.1	HD-GYP domain-containing protein	-
PAT9B_RS19080	4128397..4128726	-	330	WP_013510909.1	HigA family addiction module antidote protein	Antitoxin
PAT9B_RS19085	4128764..4129072	-	309	WP_013510910.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAT9B_RS19090	4129185..4130144	-	960	WP_013510911.1	2-hydroxyacid dehydrogenase	-
PAT9B_RS19095	4130125..4131519	-	1395	WP_013510912.1	MFS transporter	-
PAT9B_RS19100	4131650..4131874	+	225	WP_013510913.1	hypothetical protein	-
PAT9B_RS19105	4131954..4132874	+	921	WP_013510914.1	sugar ABC transporter substrate-binding protein	-
PAT9B_RS19110	4132918..4133424	-	507	WP_013510915.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12090.79 Da
• Isoelectric Point: 9.8505

>T25031 WP_013510910.1 NC_014837:c4129072-4128764 [Pantoea sp. At-9b]
MAGSIENFRDDWLQSFFVYGTPHKCIPANIESALARKLDIINAAKNYQDLRAPPGNRLEMLQPPLQRYSSIRVNGQYRLI
FQWDEGKARHLYLDPHRYKYHR

Nucleotide

• Length: 309 bp

>T25031 NC_014837:c4129072-4128764 [Pantoea sp. At-9b]
ATGGCGGGTTCAATTGAGAATTTCAGGGATGACTGGCTTCAATCATTTTTTGTGTATGGCACGCCACACAAATGTATACC
CGCCAATATTGAGTCTGCGCTGGCGCGCAAACTCGACATCATCAATGCGGCTAAAAACTATCAGGATTTACGTGCACCAC
CTGGTAATCGTTTGGAGATGCTGCAACCTCCTTTGCAGAGGTATTCATCGATAAGAGTTAACGGGCAATACCGCCTCATT
TTTCAGTGGGATGAGGGCAAGGCTCGGCATCTCTATCTCGATCCACATCGTTATAAATATCATCGATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12666.50 Da
• Isoelectric Point: 7.4027

>AT25031 WP_013510909.1 NC_014837:c4128726-4128397 [Pantoea sp. At-9b]
MKQAMRKPTSVGDVLQYEFLEPLNLKVNDLAVMLNVHRNTVSALVNNNRKLTTEMAYRLAIVFATSVDFWLDLQRHLDRW
EVENDARTQEELSRVTKLGTFLSQHHQVA

Nucleotide

• Length: 330 bp

>AT25031 NC_014837:c4128726-4128397 [Pantoea sp. At-9b]
ATGAAACAGGCAATGCGTAAGCCGACCAGCGTCGGTGATGTACTTCAGTACGAGTTTCTCGAACCCCTGAACCTGAAAGT
GAATGACTTGGCGGTCATGCTTAATGTGCACCGCAATACCGTGAGTGCGTTAGTGAATAACAATCGTAAATTGACGACAG
AAATGGCCTATCGCCTTGCAATCGTTTTTGCCACATCGGTAGATTTCTGGCTGGATCTGCAACGCCATCTTGATCGCTGG
GAAGTGGAAAACGACGCCCGCACGCAGGAAGAACTGAGTCGTGTCACCAAACTGGGAACCTTCCTCAGCCAACACCATCA
GGTGGCGTGA

Structures

Toxin

• AlphaFold DB: E6WH88