Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3969507..3970104 Replicon chromosome
Accession NZ_CP100120
Organism Klebsiella pneumoniae strain GZKP13

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIK98_RS19645 Protein ID WP_004142563.1
Coordinates 3969787..3970104 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIK98_RS19640 Protein ID WP_004142561.1
Coordinates 3969507..3969794 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIK98_RS19610 (3965587) 3965587..3965835 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIK98_RS19615 (3965853) 3965853..3966194 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
NIK98_RS19620 (3966225) 3966225..3967340 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIK98_RS19625 (3967520) 3967520..3968101 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
NIK98_RS19630 (3968101) 3968101..3968469 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIK98_RS19635 (3968589) 3968589..3969242 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIK98_RS19640 (3969507) 3969507..3969794 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIK98_RS19645 (3969787) 3969787..3970104 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIK98_RS19650 (3970289) 3970289..3971332 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIK98_RS19655 (3972002) 3972002..3972868 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIK98_RS19660 (3972977) 3972977..3974404 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250244 WP_004142563.1 NZ_CP100120:c3970104-3969787 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250244 WP_004142561.1 NZ_CP100120:c3969794-3969507 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References