Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3934471..3935068 Replicon chromosome
Accession NZ_CP100114
Organism Klebsiella pneumoniae strain HSKP1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL09_RS19200 Protein ID WP_004142563.1
Coordinates 3934751..3935068 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL09_RS19195 Protein ID WP_004142561.1
Coordinates 3934471..3934758 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL09_RS19165 (3930551) 3930551..3930799 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL09_RS19170 (3930817) 3930817..3931158 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
NIL09_RS19175 (3931189) 3931189..3932304 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL09_RS19180 (3932484) 3932484..3933065 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
NIL09_RS19185 (3933065) 3933065..3933433 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL09_RS19190 (3933553) 3933553..3934206 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL09_RS19195 (3934471) 3934471..3934758 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL09_RS19200 (3934751) 3934751..3935068 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL09_RS19205 (3935253) 3935253..3936296 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL09_RS19210 (3936966) 3936966..3937832 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL09_RS19215 (3937941) 3937941..3939368 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250213 WP_004142563.1 NZ_CP100114:c3935068-3934751 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250213 WP_004142561.1 NZ_CP100114:c3934758-3934471 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References