Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3985658..3986255 Replicon chromosome
Accession NZ_CP100110
Organism Klebsiella pneumoniae strain HSKP104

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL12_RS19490 Protein ID WP_004142563.1
Coordinates 3985938..3986255 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL12_RS19485 Protein ID WP_004142561.1
Coordinates 3985658..3985945 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL12_RS19455 (3981738) 3981738..3981986 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL12_RS19460 (3982004) 3982004..3982345 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
NIL12_RS19465 (3982376) 3982376..3983491 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL12_RS19470 (3983671) 3983671..3984252 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
NIL12_RS19475 (3984252) 3984252..3984620 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL12_RS19480 (3984740) 3984740..3985393 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL12_RS19485 (3985658) 3985658..3985945 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL12_RS19490 (3985938) 3985938..3986255 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL12_RS19495 (3986440) 3986440..3987483 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL12_RS19500 (3988153) 3988153..3989019 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL12_RS19505 (3989128) 3989128..3990555 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250195 WP_004142563.1 NZ_CP100110:c3986255-3985938 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250195 WP_004142561.1 NZ_CP100110:c3985945-3985658 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References