Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1503622..1504219 Replicon chromosome
Accession NZ_CP100102
Organism Klebsiella pneumoniae strain HSKP33

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL14_RS07370 Protein ID WP_004142563.1
Coordinates 1503902..1504219 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL14_RS07365 Protein ID WP_004142561.1
Coordinates 1503622..1503909 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL14_RS07335 (1499702) 1499702..1499950 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL14_RS07340 (1499968) 1499968..1500309 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
NIL14_RS07345 (1500340) 1500340..1501455 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL14_RS07350 (1501635) 1501635..1502216 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
NIL14_RS07355 (1502216) 1502216..1502584 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL14_RS07360 (1502704) 1502704..1503357 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL14_RS07365 (1503622) 1503622..1503909 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL14_RS07370 (1503902) 1503902..1504219 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL14_RS07375 (1504404) 1504404..1505447 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL14_RS07380 (1506117) 1506117..1506983 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL14_RS07385 (1507092) 1507092..1508519 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250150 WP_004142563.1 NZ_CP100102:c1504219-1503902 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250150 WP_004142561.1 NZ_CP100102:c1503909-1503622 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References