Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3985652..3986249 Replicon chromosome
Accession NZ_CP100098
Organism Klebsiella pneumoniae strain HSKP43

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL15_RS19490 Protein ID WP_004142563.1
Coordinates 3985932..3986249 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL15_RS19485 Protein ID WP_004142561.1
Coordinates 3985652..3985939 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL15_RS19455 (3981732) 3981732..3981980 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL15_RS19460 (3981998) 3981998..3982339 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
NIL15_RS19465 (3982370) 3982370..3983485 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL15_RS19470 (3983665) 3983665..3984246 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
NIL15_RS19475 (3984246) 3984246..3984614 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL15_RS19480 (3984734) 3984734..3985387 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL15_RS19485 (3985652) 3985652..3985939 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL15_RS19490 (3985932) 3985932..3986249 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL15_RS19495 (3986434) 3986434..3987477 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL15_RS19500 (3988147) 3988147..3989013 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL15_RS19505 (3989122) 3989122..3990549 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250138 WP_004142563.1 NZ_CP100098:c3986249-3985932 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250138 WP_004142561.1 NZ_CP100098:c3985939-3985652 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References