Detailed information of TA system TA025012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 3295483..3295988
• Replicon: chromosome
• Accession: NC_014830
• Organism: Intrasporangium calvum DSM 43043

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6SBE3
• Locus tag: INTCA_RS15055
• Protein ID: WP_013493783.1
• Coordinates: 3295483..3295749 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: INTCA_RS15060
• Protein ID: WP_041308866.1
• Coordinates: 3295746..3295988 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INTCA_RS15030	3290693..3291151	-	459	WP_148236625.1	hypothetical protein	-
INTCA_RS15040	3291833..3293278	-	1446	Protein_2961	IS1380 family transposase	-
INTCA_RS15045	3294194..3294705	+	512	Protein_2962	SigE family RNA polymerase sigma factor	-
INTCA_RS19900	3294709..3294882	+	174	WP_013493781.1	hypothetical protein	-
INTCA_RS15050	3295043..3295378	+	336	WP_013493782.1	hypothetical protein	-
INTCA_RS15055	3295483..3295749	-	267	WP_013493783.1	Txe/YoeB family addiction module toxin	Toxin
INTCA_RS15060	3295746..3295988	-	243	WP_041308866.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
INTCA_RS15065	3296661..3296942	+	282	WP_013493785.1	hypothetical protein	-
INTCA_RS15070	3297179..3297913	-	735	WP_148236627.1	hypothetical protein	-
INTCA_RS18800	3298294..3298854	-	561	WP_052338031.1	IS110 family transposase	-
INTCA_RS19230	3298931..3299803	-	873	WP_169312926.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3285009..3298084	13075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10250.82 Da
• Isoelectric Point: 9.7537

>T25012 WP_013493783.1 NC_014830:c3295749-3295483 [Intrasporangium calvum DSM 43043]
VSAWTLIYSRQAQKDAKKLASSGLKTKAQRLLDVISEDPFTTPPRYEKLVGDLAGCYSRRINIQHRLVYEVLPDQHVVHI
LRMWTHYE

Nucleotide

• Length: 267 bp

>T25012 NC_014830:c3295749-3295483 [Intrasporangium calvum DSM 43043]
GTGAGCGCCTGGACGCTGATCTATTCGCGCCAAGCTCAGAAGGACGCCAAGAAGCTGGCATCCTCCGGGTTGAAGACCAA
GGCCCAGCGCCTGCTCGACGTCATCAGCGAGGATCCATTCACCACGCCGCCGCGGTACGAGAAACTGGTCGGCGACCTGG
CGGGCTGCTACTCCCGGCGCATCAACATCCAACACCGCCTCGTCTACGAGGTCCTTCCCGACCAGCACGTCGTCCACATC
CTGCGCATGTGGACGCACTACGAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8614.50 Da
• Isoelectric Point: 3.8807

>AT25012 WP_041308866.1 NC_014830:c3295988-3295746 [Intrasporangium calvum DSM 43043]
VTAVSATTARANLYRLIDQVNDESEPLTITGQRGNAVLVGEEDWQAIQETLFLASVPGLAQSIREARAEGIEAGSTELDW

Nucleotide

• Length: 243 bp

>AT25012 NC_014830:c3295988-3295746 [Intrasporangium calvum DSM 43043]
GTGACTGCAGTGAGCGCGACGACGGCACGGGCCAACCTGTACCGCCTGATCGACCAGGTCAACGACGAGTCTGAGCCGCT
GACCATTACCGGGCAGCGGGGCAACGCGGTGCTGGTCGGGGAGGAGGACTGGCAGGCGATCCAGGAGACGCTGTTCTTGG
CCTCCGTCCCGGGTCTGGCCCAGTCCATCCGCGAGGCCCGCGCCGAGGGTATCGAGGCCGGGTCCACCGAGCTCGACTGG
TGA

Structures

Toxin

• AlphaFold DB: E6SBE3