Detailed information of TA system TA025011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3218679..3219271
• Replicon: chromosome
• Accession: NC_014830
• Organism: Intrasporangium calvum DSM 43043

Toxin (Protein)

• Gene name: graT
• Uniprot ID: E6SAT5
• Locus tag: INTCA_RS14665
• Protein ID: WP_013493708.1
• Coordinates: 3218990..3219271 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: INTCA_RS14660
• Protein ID: WP_013493707.1
• Coordinates: 3218679..3218972 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INTCA_RS14630	3213837..3214844	-	1008	WP_013493702.1	DUF4037 domain-containing protein	-
INTCA_RS14635	3215092..3215457	+	366	WP_052338024.1	hypothetical protein	-
INTCA_RS14640	3215417..3216163	+	747	WP_148236612.1	phosphatase PAP2 family protein	-
INTCA_RS14645	3216168..3216698	+	531	WP_013493704.1	DUF2127 domain-containing protein	-
INTCA_RS18785	3216740..3217714	-	975	WP_169312924.1	ATP-binding protein	-
INTCA_RS19895	3218175..3218342	+	168	WP_169312925.1	hypothetical protein	-
INTCA_RS14660	3218679..3218972	-	294	WP_013493707.1	HigA family addiction module antidote protein	Antitoxin
INTCA_RS14665	3218990..3219271	-	282	WP_013493708.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INTCA_RS14670	3220151..3220708	+	558	WP_013493709.1	hypothetical protein	-
INTCA_RS14675	3220708..3221559	+	852	WP_013493710.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
INTCA_RS14685	3222501..3222725	+	225	WP_013493711.1	hypothetical protein	-
INTCA_RS14690	3223105..3223566	+	462	WP_041307791.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10940.43 Da
• Isoelectric Point: 8.0496

>T25011 WP_013493708.1 NC_014830:c3219271-3218990 [Intrasporangium calvum DSM 43043]
VIRSFSNTMTERLWSRERTKKLDPRIERTALRKLVLLDAAETLDDLRVPPGNRLEALRGDRDGQHSIRINQQWRICFTWT
PAGPEDVEIVDYH

Nucleotide

• Length: 282 bp

>T25011 NC_014830:c3219271-3218990 [Intrasporangium calvum DSM 43043]
GTGATCAGGTCGTTCTCGAACACCATGACTGAACGGCTGTGGTCACGAGAACGCACCAAGAAGCTCGACCCGCGGATCGA
GCGGACCGCACTACGCAAGCTCGTCCTGCTCGATGCGGCCGAGACCCTCGACGACCTGCGGGTCCCACCCGGCAACCGGC
TCGAAGCCCTGCGCGGCGACCGGGACGGACAACACAGCATCCGAATCAACCAGCAGTGGCGGATCTGCTTCACCTGGACA
CCCGCAGGGCCCGAAGACGTCGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11085.74 Da
• Isoelectric Point: 7.0736

>AT25011 WP_013493707.1 NC_014830:c3218972-3218679 [Intrasporangium calvum DSM 43043]
MATIRPIHPGEVLLEDFLTPLEVTQHHLAVSIGVPPRRINEIVHGKRRITADTALRLARYFGTTDRFWLNLQTRYDLEIE
KDHLGAALDQIHPLQSA

Nucleotide

• Length: 294 bp

>AT25011 NC_014830:c3218972-3218679 [Intrasporangium calvum DSM 43043]
ATGGCGACCATCCGGCCCATCCACCCGGGGGAGGTCCTCCTCGAGGACTTCCTGACCCCGCTGGAGGTCACCCAGCACCA
CCTCGCCGTCTCGATCGGTGTCCCGCCCCGGCGGATCAACGAGATCGTCCACGGAAAGCGCCGGATCACCGCCGACACCG
CGCTTCGCCTGGCCCGCTACTTCGGCACCACCGACCGGTTCTGGCTCAACCTGCAAACCCGCTACGACCTCGAGATCGAG
AAGGACCACCTCGGCGCCGCCCTCGACCAGATCCATCCCCTGCAGAGCGCCTGA

Structures

Toxin

• AlphaFold DB: E6SAT5