Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4568414..4569011 Replicon chromosome
Accession NZ_CP100087
Organism Klebsiella pneumoniae strain HSKP8

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL17_RS22215 Protein ID WP_004142563.1
Coordinates 4568694..4569011 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL17_RS22210 Protein ID WP_004142561.1
Coordinates 4568414..4568701 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL17_RS22180 (4564494) 4564494..4564742 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL17_RS22185 (4564760) 4564760..4565101 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
NIL17_RS22190 (4565132) 4565132..4566247 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL17_RS22195 (4566427) 4566427..4567008 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
NIL17_RS22200 (4567008) 4567008..4567376 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL17_RS22205 (4567496) 4567496..4568149 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL17_RS22210 (4568414) 4568414..4568701 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL17_RS22215 (4568694) 4568694..4569011 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL17_RS22220 (4569196) 4569196..4570239 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL17_RS22225 (4570909) 4570909..4571775 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL17_RS22230 (4571884) 4571884..4573311 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250102 WP_004142563.1 NZ_CP100087:c4569011-4568694 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250102 WP_004142561.1 NZ_CP100087:c4568701-4568414 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References