Detailed information of TA system TA025009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 933636..934152
• Replicon: chromosome
• Accession: NC_014830
• Organism: Intrasporangium calvum DSM 43043

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6SBQ4
• Locus tag: INTCA_RS04290
• Protein ID: WP_013491704.1
• Coordinates: 933636..933893 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E6SBQ5
• Locus tag: INTCA_RS04295
• Protein ID: WP_013491705.1
• Coordinates: 933895..934152 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INTCA_RS04260	928708..929676	+	969	WP_013491699.1	MBL fold metallo-hydrolase	-
INTCA_RS04265	929695..931437	+	1743	WP_013491700.1	CocE/NonD family hydrolase	-
INTCA_RS04270	931434..931787	+	354	Protein_845	acetyl-CoA acetyltransferase	-
INTCA_RS04275	931796..932491	+	696	WP_013491702.1	3-oxoacid CoA-transferase subunit A	-
INTCA_RS04280	932491..933123	+	633	WP_013491703.1	3-oxoacid CoA-transferase subunit B	-
INTCA_RS04290	933636..933893	-	258	WP_013491704.1	Txe/YoeB family addiction module toxin	Toxin
INTCA_RS04295	933895..934152	-	258	WP_013491705.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
INTCA_RS04300	934614..934931	+	318	WP_013491706.1	hypothetical protein	-
INTCA_RS04305	935154..935912	+	759	WP_013491707.1	aquaporin family protein	-
INTCA_RS04310	935985..937199	+	1215	WP_013491708.1	formaldehyde dehydrogenase, glutathione-independent	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10215.55 Da
• Isoelectric Point: 7.2752

>T25009 WP_013491704.1 NC_014830:c933893-933636 [Intrasporangium calvum DSM 43043]
VLLVWDENAWEDYLWWQAQDRRVLKRIKVLLADIQRNGNEGIGKPEALKHDFAGYWSRRITDEHRLVYKVVDDEVRTAAC
RYHYG

Nucleotide

• Length: 258 bp

>T25009 NC_014830:c933893-933636 [Intrasporangium calvum DSM 43043]
GTGCTGCTCGTCTGGGACGAGAACGCGTGGGAGGACTATCTCTGGTGGCAAGCGCAAGACCGCAGGGTCCTGAAGAGAAT
CAAGGTGCTGCTCGCTGACATCCAACGCAACGGCAACGAGGGCATCGGCAAGCCGGAAGCGCTCAAGCATGACTTCGCGG
GCTACTGGTCCAGACGCATCACCGATGAGCACCGCCTCGTGTACAAAGTCGTCGACGACGAGGTCCGGACCGCTGCCTGT
CGCTACCACTACGGATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9713.87 Da
• Isoelectric Point: 4.5822

>AT25009 WP_013491705.1 NC_014830:c934152-933895 [Intrasporangium calvum DSM 43043]
MKTMSYTESRARYAEVLDGVANDREEVVITRAGHEPVVIVSLEDYESLRETAYLMRSPANARRLLDAMERLEADRSKGHD
LIETD

Nucleotide

• Length: 258 bp

>AT25009 NC_014830:c934152-933895 [Intrasporangium calvum DSM 43043]
ATGAAGACGATGAGTTACACCGAATCCCGGGCTCGCTACGCCGAGGTACTCGACGGGGTTGCCAACGACCGTGAGGAGGT
CGTCATCACCCGGGCGGGACATGAGCCCGTAGTCATCGTCTCCCTCGAGGACTACGAGTCCCTCCGGGAGACGGCCTACC
TGATGCGTTCACCCGCCAATGCCCGACGCCTCCTCGACGCCATGGAGCGTCTGGAGGCCGATCGCAGTAAAGGTCATGAC
CTGATCGAGACCGACTGA

Structures

Toxin

• AlphaFold DB: E6SBQ4

Antitoxin

• AlphaFold DB: E6SBQ5