Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4022591..4023188 Replicon chromosome
Accession NZ_CP100084
Organism Klebsiella pneumoniae strain HSKP86

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL18_RS19670 Protein ID WP_004142563.1
Coordinates 4022871..4023188 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL18_RS19665 Protein ID WP_004142561.1
Coordinates 4022591..4022878 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL18_RS19635 (4018671) 4018671..4018919 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL18_RS19640 (4018937) 4018937..4019278 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
NIL18_RS19645 (4019309) 4019309..4020424 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL18_RS19650 (4020604) 4020604..4021185 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
NIL18_RS19655 (4021185) 4021185..4021553 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL18_RS19660 (4021673) 4021673..4022326 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL18_RS19665 (4022591) 4022591..4022878 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL18_RS19670 (4022871) 4022871..4023188 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL18_RS19675 (4023373) 4023373..4024416 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL18_RS19680 (4025086) 4025086..4025952 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL18_RS19685 (4026061) 4026061..4027488 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250080 WP_004142563.1 NZ_CP100084:c4023188-4022871 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T250080 NZ_CP100084:c4023188-4022871 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250080 WP_004142561.1 NZ_CP100084:c4022878-4022591 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT250080 NZ_CP100084:c4022878-4022591 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References