Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3977895..3978492 Replicon chromosome
Accession NZ_CP100072
Organism Klebsiella pneumoniae strain RJKP41

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag NIL01_RS19450 Protein ID WP_004142563.1
Coordinates 3978175..3978492 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag NIL01_RS19445 Protein ID WP_004142561.1
Coordinates 3977895..3978182 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIL01_RS19415 (3973975) 3973975..3974223 + 249 WP_020325243.1 DUF1158 domain-containing protein -
NIL01_RS19420 (3974241) 3974241..3974582 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
NIL01_RS19425 (3974613) 3974613..3975728 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
NIL01_RS19430 (3975908) 3975908..3976489 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
NIL01_RS19435 (3976489) 3976489..3976857 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
NIL01_RS19440 (3976977) 3976977..3977630 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
NIL01_RS19445 (3977895) 3977895..3978182 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
NIL01_RS19450 (3978175) 3978175..3978492 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NIL01_RS19455 (3978677) 3978677..3979720 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
NIL01_RS19460 (3980390) 3980390..3981256 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
NIL01_RS19465 (3981365) 3981365..3982792 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T250039 WP_004142563.1 NZ_CP100072:c3978492-3978175 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT250039 WP_004142561.1 NZ_CP100072:c3978182-3977895 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References