Detailed information of TA system TA024999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-couple_hipB
• Location: 1143821..1144497
• Replicon: chromosome
• Accession: NC_014815
• Organism: Micromonospora sp. L5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1C6T5J3
• Locus tag: ML5_RS05750
• Protein ID: WP_013474046.1
• Coordinates: 1144135..1144497 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ML5_RS05745
• Protein ID: WP_013284102.1
• Coordinates: 1143821..1144138 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML5_RS05705	1139016..1139651	+	636	WP_013284094.1	septum formation initiator family protein	-
ML5_RS05710	1139675..1140232	+	558	WP_013284095.1	DUF501 domain-containing protein	-
ML5_RS05715	1140234..1140743	+	510	WP_013284096.1	amino-acid N-acetyltransferase	-
ML5_RS05720	1140786..1141715	+	930	WP_013474042.1	Ppx/GppA family phosphatase	-
ML5_RS05725	1141818..1141997	-	180	WP_013284098.1	hypothetical protein	-
ML5_RS05730	1142098..1142385	+	288	WP_013474043.1	hypothetical protein	-
ML5_RS05735	1142450..1142782	+	333	WP_030271259.1	PadR family transcriptional regulator	-
ML5_RS05740	1142779..1143738	+	960	WP_013474045.1	hypothetical protein	-
ML5_RS05745	1143821..1144138	-	318	WP_013284102.1	helix-turn-helix domain-containing protein	Antitoxin
ML5_RS05750	1144135..1144497	-	363	WP_013474046.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ML5_RS05755	1144675..1145415	+	741	WP_013474047.1	dienelactone hydrolase family protein	-
ML5_RS05760	1145435..1146148	+	714	WP_013474048.1	uracil-DNA glycosylase	-
ML5_RS05765	1146169..1146900	+	732	WP_013474049.1	DUF4129 domain-containing protein	-
ML5_RS05770	1146897..1147481	+	585	WP_013284107.1	hypothetical protein	-
ML5_RS05775	1147481..1148479	+	999	WP_013474050.1	MoxR family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13581.42 Da
• Isoelectric Point: 6.0857

>T24999 WP_013474046.1 NC_014815:c1144497-1144135 [Micromonospora sp. L5]
VVAGDWDVYLVDEVRQWLDTLDASTHARVVQAIDLLAEHGPGLGRPLVDTIHGSSMANLKELRPGTVRILFAFDPWRSSI
LLVAGDKSGRWKTWYQEAIPVAEHRYELYLKDRAREEGKA

Nucleotide

• Length: 363 bp

>T24999 NC_014815:c1144497-1144135 [Micromonospora sp. L5]
GTGGTGGCTGGAGACTGGGACGTCTACCTGGTCGACGAGGTGCGGCAGTGGCTCGACACTCTCGACGCGTCCACCCATGC
GCGAGTGGTGCAGGCGATCGACCTCCTGGCCGAGCACGGACCAGGACTCGGACGACCCCTTGTCGACACCATCCACGGTT
CGTCGATGGCGAACTTGAAGGAGCTCCGGCCGGGCACCGTGCGTATCCTTTTCGCGTTCGACCCGTGGCGCTCGAGCATC
CTCCTGGTCGCCGGCGACAAGTCCGGCCGGTGGAAGACGTGGTATCAGGAGGCCATTCCCGTCGCCGAGCACCGCTACGA
GCTCTACCTCAAGGACCGGGCACGAGAGGAGGGGAAGGCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11399.80 Da
• Isoelectric Point: 6.7267

>AT24999 WP_013284102.1 NC_014815:c1144138-1143821 [Micromonospora sp. L5]
MSDYSRWQDIRADHVARVGGEDAVDAGKQELLAEVIGHRLAEVRRARGLTQQQVADRMGVTKGRVSQIEQGKISGQEVVA
RFAAALGGRLHQAIYFDDGDIAAIA

Nucleotide

• Length: 318 bp

>AT24999 NC_014815:c1144138-1143821 [Micromonospora sp. L5]
ATGAGCGACTACTCCCGGTGGCAGGACATCCGCGCCGACCACGTCGCCCGGGTCGGCGGGGAAGATGCGGTCGACGCGGG
TAAGCAGGAACTCCTCGCCGAGGTCATCGGTCACCGCCTCGCGGAGGTACGGCGAGCGCGCGGCCTGACCCAGCAGCAGG
TCGCCGACCGGATGGGCGTCACCAAGGGACGGGTCTCCCAGATCGAGCAGGGAAAGATCTCCGGACAGGAAGTGGTGGCC
CGGTTCGCCGCGGCGCTGGGTGGCCGGCTCCACCAGGCGATCTACTTCGACGACGGTGACATCGCCGCCATCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1C6T5J3