Detailed information of TA system TA024990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 380783..381389
• Replicon: chromosome
• Accession: NC_014762
• Organism: Sulfuricurvum kujiense DSM 16994

Toxin (Protein)

• Gene name: graT
• Uniprot ID: E4TZ50
• Locus tag: SULKU_RS01955
• Protein ID: WP_013459246.1
• Coordinates: 380783..381079 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SULKU_RS01960
• Protein ID: WP_013459247.1
• Coordinates: 381090..381389 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULKU_RS01940	376570..377973	-	1404	WP_013459243.1	DASS family sodium-coupled anion symporter	-
SULKU_RS01945	378140..378679	+	540	WP_013459244.1	nucleotide exchange factor GrpE	-
SULKU_RS01950	378801..380678	+	1878	WP_013459245.1	molecular chaperone DnaK	-
SULKU_RS01955	380783..381079	+	297	WP_013459246.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SULKU_RS01960	381090..381389	+	300	WP_013459247.1	HigA family addiction module antidote protein	Antitoxin
SULKU_RS01965	381401..382633	+	1233	WP_013459248.1	glutamate-5-semialdehyde dehydrogenase	-
SULKU_RS01970	382630..383202	+	573	WP_013459249.1	ATP-binding cassette domain-containing protein	-
SULKU_RS01975	383195..383851	-	657	WP_049766996.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
SULKU_RS01980	383886..385040	-	1155	WP_013459251.1	hypothetical protein	-
SULKU_RS01985	385037..385711	-	675	WP_013459252.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
SULKU_RS01990	385708..385950	-	243	WP_013459253.1	phosphoribosylformylglycinamidine synthase subunit PurS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11233.78 Da
• Isoelectric Point: 5.8405

>T24990 WP_013459246.1 NC_014762:380783-381079 [Sulfuricurvum kujiense DSM 16994]
MIISFADQATSDIFNGIESKQARKALPMELWKSASRKLDQIDSVIMLDELKVPPGNRLEKLRGSRENQMSIRINDQYRIC
FTWSDTGAVNVEITDYHS

Nucleotide

• Length: 297 bp

>T24990 NC_014762:380783-381079 [Sulfuricurvum kujiense DSM 16994]
ATGATAATTTCGTTTGCGGATCAAGCTACATCGGATATATTTAACGGCATAGAGAGCAAACAAGCTCGTAAAGCTCTTCC
GATGGAACTATGGAAAAGTGCATCTCGAAAACTTGATCAGATCGATTCGGTCATCATGTTGGATGAATTAAAGGTTCCCC
CCGGAAATCGTCTCGAAAAACTGCGCGGGAGCAGAGAAAATCAAATGAGTATCCGTATCAACGATCAGTACCGTATCTGT
TTTACATGGAGTGACACGGGTGCGGTAAATGTAGAAATAACCGATTATCACTCATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11364.15 Da
• Isoelectric Point: 7.3171

>AT24990 WP_013459247.1 NC_014762:381090-381389 [Sulfuricurvum kujiense DSM 16994]
MNIPTHRAPTHPGEMLLTEFLEPMGITQRTLADAIHVPYQRVNEIINQKRGITPATALRLAKYFGMSADFWLNLQIRWDL
YRSMQSEAKEIEAIQPLAS

Nucleotide

• Length: 300 bp

>AT24990 NC_014762:381090-381389 [Sulfuricurvum kujiense DSM 16994]
ATGAATATCCCAACCCACAGAGCACCTACCCATCCGGGTGAAATGCTCCTCACCGAGTTTTTAGAACCGATGGGGATAAC
GCAACGGACGTTAGCCGATGCGATTCATGTCCCCTATCAGCGTGTCAATGAGATCATCAATCAAAAGCGGGGGATTACTC
CGGCTACGGCACTTCGCCTTGCAAAATATTTCGGGATGTCGGCGGATTTTTGGCTGAATCTCCAAATCCGATGGGATTTA
TATCGATCCATGCAGTCGGAAGCCAAAGAGATTGAAGCAATTCAGCCTTTGGCATCATAA

Structures

Toxin

• AlphaFold DB: E4TZ50