Detailed information of TA system TA024986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 46448..47040
• Replicon: plasmid pSULKU02
• Accession: NC_014755
• Organism: Sulfuricurvum kujiense DSM 16994

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E4U3V5
• Locus tag: SULKU_RS13690
• Protein ID: WP_013449982.1
• Coordinates: 46726..47040 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SULKU_RS13685
• Protein ID: WP_013449981.1
• Coordinates: 46448..46726 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULKU_RS13665	41693..42289	-	597	WP_013449978.1	TetR/AcrR family transcriptional regulator	-
SULKU_RS14670	42417..45446	-	3030	WP_013449979.1	Tn3 family transposase	-
SULKU_RS13675	45602..46219	+	618	WP_013449888.1	recombinase family protein	-
SULKU_RS13680	46245..46430	-	186	WP_013449980.1	BrnA antitoxin family protein	-
SULKU_RS13685	46448..46726	-	279	WP_013449981.1	helix-turn-helix domain-containing protein	Antitoxin
SULKU_RS13690	46726..47040	-	315	WP_013449982.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SULKU_RS13695	47317..47508	-	192	WP_013449983.1	hypothetical protein	-
SULKU_RS13700	47550..47903	-	354	WP_013449984.1	DNA-binding protein	-
SULKU_RS13705	47907..48200	-	294	WP_013449985.1	type II toxin-antitoxin system HigB family toxin	-
SULKU_RS13710	48338..50284	-	1947	WP_013449986.1	PAS domain S-box protein	-
SULKU_RS13715	50669..51265	-	597	WP_013449987.1	pentapeptide repeat-containing protein	-
SULKU_RS13720	51262..51654	-	393	WP_013449988.1	thioredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71513	71513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11993.87 Da
• Isoelectric Point: 9.5913

>T24986 WP_013449982.1 NC_014755:c47040-46726 [Sulfuricurvum kujiense DSM 16994]
MEIAETTIFTREITRILSDEEYKELQTFLVEHPKSGAVIKGSGGLRKIRWSIKNSGKSGGIRNIYYYHEENSLILMIYVY
EKSKTSDLSPKQIEMLRKTFMGGL

Nucleotide

• Length: 315 bp

>T24986 NC_014755:c47040-46726 [Sulfuricurvum kujiense DSM 16994]
ATGGAAATAGCCGAAACTACGATCTTTACCCGTGAAATCACCCGTATTCTCTCTGATGAGGAATACAAAGAGCTTCAAAC
TTTTTTGGTGGAACACCCTAAAAGCGGAGCGGTGATCAAAGGATCAGGGGGTTTGCGTAAAATTCGATGGTCTATTAAAA
ACAGCGGTAAAAGTGGTGGTATCCGAAATATCTACTACTATCATGAGGAAAATAGTCTTATCCTTATGATCTATGTGTAT
GAAAAAAGTAAAACATCCGATTTATCCCCAAAACAAATCGAGATGCTCAGAAAAACTTTCATGGGAGGCTTATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10292.84 Da
• Isoelectric Point: 8.7555

>AT24986 WP_013449981.1 NC_014755:c46726-46448 [Sulfuricurvum kujiense DSM 16994]
MEDAMFNDLLASVGEAKEILGKTKKPSRSFMIDEPDAKSIRARLKMTQDEFAGLLNISVNTLRNWEQSRRKPEGPARVLL
GIAATHPEVFAH

Nucleotide

• Length: 279 bp

>AT24986 NC_014755:c46726-46448 [Sulfuricurvum kujiense DSM 16994]
ATGGAAGATGCAATGTTTAATGACTTGCTTGCAAGTGTTGGTGAAGCAAAAGAGATTCTTGGTAAGACAAAAAAACCATC
ACGTTCATTTATGATCGATGAACCGGATGCCAAGTCTATACGAGCTCGTTTAAAAATGACACAAGATGAATTTGCAGGAC
TTTTAAATATTAGTGTAAATACACTAAGAAACTGGGAACAATCACGTCGTAAACCAGAAGGTCCGGCTCGTGTATTACTA
GGAATTGCAGCTACTCATCCGGAAGTATTTGCTCACTAA

Structures

Toxin

• AlphaFold DB: E4U3V5