Detailed information of TA system TA024983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2214333..2214934
• Replicon: chromosome
• Accession: NC_014733
• Organism: Methylovorus sp. MP688

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MPQ_RS10510
• Protein ID: WP_013442921.1
• Coordinates: 2214333..2214644 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MPQ_RS10515
• Protein ID: WP_013442922.1
• Coordinates: 2214647..2214934 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPQ_RS10485	2209643..2210068	-	426	Protein_2068	site-specific integrase	-
MPQ_RS10490	2210241..2210714	-	474	WP_148225290.1	hypothetical protein	-
MPQ_RS10495	2210783..2211106	+	324	WP_049821903.1	hypothetical protein	-
MPQ_RS10500	2211227..2211463	-	237	WP_013442918.1	hypothetical protein	-
MPQ_RS10505	2211789..2214188	+	2400	WP_013442920.1	catalase	-
MPQ_RS10510	2214333..2214644	+	312	WP_013442921.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPQ_RS10515	2214647..2214934	+	288	WP_013442922.1	helix-turn-helix domain-containing protein	Antitoxin
MPQ_RS10520	2214985..2215944	+	960	WP_013442923.1	hypothetical protein	-
MPQ_RS10525	2216152..2218704	+	2553	WP_013442924.1	MCP four helix bundle domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-77)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11823.72 Da
• Isoelectric Point: 8.3879

>T24983 WP_013442921.1 NC_014733:2214333-2214644 [Methylovorus sp. MP688]
MIFIETPTFTRLITDLLPDEEYGKLQEELARQPDAGDLIKNGGGIRKLRWKRTGTGKSGGIRVIYYWITDDDQILMLVAY
PKNVKDNLTDKETAILRKLVKEL

Nucleotide

• Length: 312 bp

>T24983 NC_014733:2214333-2214644 [Methylovorus sp. MP688]
ATGATTTTCATTGAAACGCCCACCTTCACCAGACTGATTACAGACCTGCTCCCCGATGAGGAATATGGCAAATTGCAAGA
AGAGCTTGCCCGCCAGCCAGATGCAGGCGATCTCATCAAGAATGGCGGAGGAATCCGCAAACTACGCTGGAAAAGAACAG
GCACTGGCAAAAGCGGCGGTATAAGGGTGATCTACTACTGGATTACCGATGACGACCAGATTTTGATGCTGGTGGCTTAC
CCCAAGAACGTAAAAGACAATCTCACAGATAAAGAAACCGCAATATTGCGCAAGTTGGTAAAGGAGCTATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10442.07 Da
• Isoelectric Point: 10.3102

>AT24983 WP_013442922.1 NC_014733:2214647-2214934 [Methylovorus sp. MP688]
MDKTLFEDLQQSLKEAVAIRRSEIAPGKVTEIHTPDAKAIRDKVGLSQTEFAQLIGVKVATLRNWEQNRRKPTGAAAALL
TIVEKEPAAALRALH

Nucleotide

• Length: 288 bp

>AT24983 NC_014733:2214647-2214934 [Methylovorus sp. MP688]
ATGGACAAAACATTGTTTGAAGATTTGCAACAAAGCCTGAAAGAAGCCGTTGCCATTCGCCGCAGCGAGATAGCGCCCGG
CAAAGTCACTGAAATCCACACGCCCGACGCCAAGGCCATCCGCGACAAAGTCGGCCTGAGCCAGACAGAGTTTGCCCAGC
TCATCGGCGTAAAAGTAGCCACATTAAGAAATTGGGAGCAGAACCGGCGCAAGCCTACGGGAGCTGCAGCGGCGTTATTG
ACGATTGTGGAGAAAGAACCTGCGGCGGCGTTGAGGGCGTTGCATTAA