Detailed information of TA system TA249721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 4868477..4868900
• Replicon: chromosome
• Accession: NZ_CP099886
• Organism: Escherichia albertii strain 222_1_EW_B

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: NIZ19_RS23945
• Protein ID: WP_228769536.1
• Coordinates: 4868781..4868900 (+)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	4868477..4868693 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NIZ19_RS23910 (4863612)	4863612..4864592	-	981	WP_164512216.1	IS110 family transposase	-
NIZ19_RS23915 (4864844)	4864844..4865377	+	534	WP_099588255.1	single-stranded DNA-binding protein	-
NIZ19_RS23920 (4865435)	4865435..4865668	+	234	WP_099588254.1	DUF905 family protein	-
NIZ19_RS23925 (4865732)	4865732..4867260	+	1529	Protein_4655	ParB/RepB/Spo0J family partition protein	-
NIZ19_RS23930 (4867315)	4867315..4867749	+	435	WP_099588253.1	conjugation system SOS inhibitor PsiB	-
NIZ19_RS23935 (4867746)	4867746..4868508	+	763	Protein_4657	plasmid SOS inhibition protein A	-
- (4868477)	4868477..4868693	+	217	NuclAT_0	-	-
- (4868477)	4868477..4868693	+	217	NuclAT_0	-	-
- (4868477)	4868477..4868693	+	217	NuclAT_0	-	-
- (4868477)	4868477..4868693	+	217	NuclAT_0	-	-
NIZ19_RS23940 (4868681)	4868681..4868833	+	153	Protein_4658	FlmC family protein	-
NIZ19_RS23945 (4868781)	4868781..4868900	+	120	WP_228769536.1	Hok/Gef family protein	Toxin
NIZ19_RS23950 (4869201)	4869201..4869494	-	294	Protein_4660	hypothetical protein	-
NIZ19_RS23955 (4869512)	4869512..4869798	+	287	Protein_4661	hypothetical protein	-
NIZ19_RS23960 (4869917)	4869917..4870738	+	822	WP_157760423.1	DUF932 domain-containing protein	-
NIZ19_RS23965 (4871035)	4871035..4871682	-	648	WP_157760422.1	transglycosylase SLT domain-containing protein	-
NIZ19_RS23970 (4871958)	4871958..4872341	+	384	WP_019842734.1	conjugal transfer relaxosome DNA-binding protein TraM	-
NIZ19_RS23975 (4872536)	4872536..4873183	+	648	WP_256877304.1	transcriptional regulator TraJ family protein	-
NIZ19_RS23980 (4873302)	4873302..4873529	+	228	WP_019842732.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / papC / papD	4821578..4873529	51951
	-	inside	Genomic island	-	vat / papC / papD	4821578..4873921	52343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4425.22 Da
• Isoelectric Point: 8.2691

>T249721 WP_228769536.1 NZ_CP099886:4868781-4868900 [Escherichia albertii]
VCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 120 bp

Antitoxin

Nucleotide

• Length: 217 bp

>AT249721 NZ_CP099886:c4868693-4868477 [Escherichia albertii]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACGACAGGATA
ACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACA
CTCACTTTTCCTGAAAATAACCCGCTCATTCAGGCTGTTTACAGGTAATCTGTGTGA

Structures

Antitoxin