Detailed information of TA system TA024969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2270430..2271163
• Replicon: chromosome
• Accession: NC_014666
• Organism: Frankia sp. EuI1c

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: FRAEUI1C_RS09225
• Protein ID: WP_013423028.1
• Coordinates: 2270430..2270852 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: E3JCS0
• Locus tag: FRAEUI1C_RS09230
• Protein ID: WP_013423029.1
• Coordinates: 2270849..2271163 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRAEUI1C_RS09190	2265459..2265896	+	438	WP_013423023.1	DUF3052 domain-containing protein	-
FRAEUI1C_RS09195	2266059..2266445	-	387	WP_157734878.1	DUF4234 domain-containing protein	-
FRAEUI1C_RS09200	2267133..2267591	+	459	WP_013423025.1	peroxiredoxin	-
FRAEUI1C_RS36055	2267861..2268304	+	444	WP_013423026.1	hypothetical protein	-
FRAEUI1C_RS37475	2268475..2269725	-	1251	WP_013423027.1	ATP-binding protein	-
FRAEUI1C_RS09225	2270430..2270852	+	423	WP_013423028.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRAEUI1C_RS09230	2270849..2271163	+	315	WP_013423029.1	helix-turn-helix transcriptional regulator	Antitoxin
FRAEUI1C_RS09235	2271498..2272729	-	1232	Protein_1823	IS481 family transposase	-
FRAEUI1C_RS40155	2273138..2273401	-	264	WP_049806859.1	hypothetical protein	-
FRAEUI1C_RS40160	2273398..2273691	-	294	WP_198318795.1	hypothetical protein	-
FRAEUI1C_RS39420	2273654..2273899	+	246	WP_157734879.1	hypothetical protein	-
FRAEUI1C_RS09245	2274031..2274696	-	666	WP_013423030.1	TetR/AcrR family transcriptional regulator	-
FRAEUI1C_RS09250	2274951..2275808	+	858	WP_013423031.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (29-126)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15389.64 Da
• Isoelectric Point: 10.3572

>T24969 WP_013423028.1 NC_014666:2270430-2270852 [Frankia inefficax]
MFSLSYSVGVSAGLSPRWTIKTTREVRDWLRALRQADPVTYRSVNVAIDMLADAGPALGRPVVDTLAGSEIKNLKELRPR
SGRTVAIRILFVFDPWSQAVLLIAGNKAGDWTGWYETAIPVAEAAYDGWLAAERKRRGGS

Nucleotide

• Length: 423 bp

>T24969 NC_014666:2270430-2270852 [Frankia inefficax]
ATGTTTAGCCTGAGCTATAGTGTCGGTGTGTCCGCAGGCTTGTCCCCTCGGTGGACGATCAAGACGACTCGTGAGGTGCG
GGACTGGCTGCGCGCGCTGCGGCAGGCTGATCCGGTTACCTACCGATCGGTGAACGTGGCGATCGACATGCTCGCCGATG
CGGGTCCGGCCCTTGGCCGGCCGGTCGTCGATACGTTGGCCGGTTCGGAGATCAAAAATCTCAAGGAGCTACGGCCGCGG
TCAGGTCGCACAGTGGCGATCCGGATTCTCTTTGTCTTCGACCCGTGGTCGCAGGCTGTGTTGCTGATTGCGGGCAACAA
GGCTGGTGACTGGACGGGCTGGTACGAAACTGCGATCCCGGTTGCCGAGGCGGCGTATGACGGATGGCTAGCGGCCGAGA
GGAAGCGGAGGGGCGGCTCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11702.87 Da
• Isoelectric Point: 4.3945

>AT24969 WP_013423029.1 NC_014666:2270849-2271163 [Frankia inefficax]
VTEFYDWDDLRAELHDGDDEALAAERARTEAWISAFHLAEERTRLGLTQRQVAESMGVSPGRVSQIENGDLDANEVATLS
RYARALGARLRIIFDYGNELHQIA

Nucleotide

• Length: 315 bp

>AT24969 NC_014666:2270849-2271163 [Frankia inefficax]
GTGACTGAGTTCTATGACTGGGACGACCTTCGCGCCGAGTTGCACGACGGCGACGATGAAGCGCTGGCTGCGGAGCGAGC
CCGTACAGAGGCTTGGATCAGTGCGTTTCACCTGGCTGAGGAGCGCACACGACTCGGTCTCACACAGCGGCAGGTAGCCG
AGTCCATGGGTGTGTCTCCCGGCCGGGTCAGCCAGATCGAAAACGGCGACCTCGACGCCAACGAGGTCGCCACCCTGAGC
CGGTATGCGCGGGCACTTGGAGCCCGACTACGGATCATCTTCGACTACGGGAACGAACTGCACCAAATTGCCTGA

Structures

Antitoxin

• AlphaFold DB: E3JCS0