Detailed information of TA system TA024967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3068967..3069580
• Replicon: chromosome
• Accession: NC_014664
• Organism: Rhodomicrobium vannielii ATCC 17100

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E3I8Q4
• Locus tag: RVAN_RS14215
• Protein ID: WP_013420404.1
• Coordinates: 3069284..3069580 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RVAN_RS14210
• Protein ID: WP_013420403.1
• Coordinates: 3068967..3069275 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVAN_RS14190	3064037..3064525	+	489	WP_013420399.1	PGDYG domain-containing protein	-
RVAN_RS14195	3064775..3065740	+	966	WP_013420400.1	IS481 family transposase	-
RVAN_RS20305	3065930..3066175	-	246	WP_210160481.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
RVAN_RS19155	3066332..3067738	-	1407	WP_013420401.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
RVAN_RS14205	3067825..3068922	-	1098	WP_013420402.1	RNA-directed DNA polymerase	-
RVAN_RS14210	3068967..3069275	-	309	WP_013420403.1	HigA family addiction module antidote protein	Antitoxin
RVAN_RS14215	3069284..3069580	-	297	WP_013420404.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RVAN_RS14220	3069644..3070009	-	366	WP_013420405.1	diversity-generating retroelement protein Avd	-
RVAN_RS19160	3070118..3073126	-	3009	WP_013420406.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
RVAN_RS14235	3073521..3073913	-	393	WP_013420407.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3019135..3101085	81950
	-	flank	IS/Tn	-	-	3064775..3065740	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11617.14 Da
• Isoelectric Point: 7.3190

>T24967 WP_013420404.1 NC_014664:c3069580-3069284 [Rhodomicrobium vannielii ATCC 17100]
VIVSFRDDWLRDFFVDDVRSKKIPPDLESRLFRKLQMLDDATADRDLRVPPSNHFEKLRGNLEGFHSIRVNKQWRLIFQW
DGGRGEATGVYLDGHIYQ

Nucleotide

• Length: 297 bp

>T24967 NC_014664:c3069580-3069284 [Rhodomicrobium vannielii ATCC 17100]
GTGATTGTAAGCTTCCGGGACGATTGGTTGCGCGATTTCTTCGTCGATGACGTCCGGTCGAAGAAAATCCCGCCCGATCT
CGAAAGCCGCCTGTTTCGCAAACTACAGATGCTCGACGACGCGACGGCGGACCGAGATTTGAGGGTTCCGCCCAGCAACC
ACTTCGAAAAGCTGCGCGGCAATCTTGAAGGCTTCCATTCCATCCGCGTCAACAAGCAATGGCGGCTTATCTTTCAATGG
GATGGCGGCCGGGGTGAAGCAACAGGGGTTTATCTGGACGGCCACATTTATCAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11430.24 Da
• Isoelectric Point: 8.5694

>AT24967 WP_013420403.1 NC_014664:c3069275-3068967 [Rhodomicrobium vannielii ATCC 17100]
MLITKRKPASPGEILTQEFMEPLGLTQSALAEAMGVPRKHVNELCNGRRAVTAPTALILARVFGNSPEFWLNVQRRCDLW
DAMHDPHERERVERAKPLTAAA

Nucleotide

• Length: 309 bp

>AT24967 NC_014664:c3069275-3068967 [Rhodomicrobium vannielii ATCC 17100]
ATGTTGATCACCAAGCGCAAGCCGGCGAGCCCCGGCGAAATCCTAACCCAGGAGTTCATGGAGCCCTTGGGGTTGACGCA
AAGCGCGCTGGCTGAGGCCATGGGCGTTCCACGCAAGCATGTGAACGAACTCTGCAATGGGCGCCGCGCCGTCACCGCTC
CCACCGCGCTCATTTTGGCCCGCGTGTTCGGCAACAGCCCCGAGTTCTGGCTGAATGTGCAGCGGCGCTGCGATCTGTGG
GACGCGATGCACGATCCGCATGAACGGGAGCGGGTCGAGCGCGCCAAGCCGCTGACGGCCGCTGCGTGA

Structures

Toxin

• AlphaFold DB: E3I8Q4