Detailed information of TA system TA024959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 6528055..6528658
• Replicon: chromosome
• Accession: NC_014640
• Organism: Achromobacter xylosoxidans A8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E3HLL6
• Locus tag: AXYL_RS30000
• Protein ID: WP_013396646.1
• Coordinates: 6528055..6528366 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXYL_RS30005
• Protein ID: WP_013396647.1
• Coordinates: 6528368..6528658 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXYL_RS29970	6523535..6523846	+	312	WP_006216228.1	50S ribosomal protein L21	-
AXYL_RS29975	6523881..6524141	+	261	WP_006216229.1	50S ribosomal protein L27	-
AXYL_RS29980	6524331..6525467	+	1137	WP_013396642.1	GTPase ObgE	-
AXYL_RS29985	6525525..6526661	+	1137	WP_013396643.1	glutamate 5-kinase	-
AXYL_RS29990	6526666..6527340	-	675	WP_013396644.1	transcriptional regulator	-
AXYL_RS29995	6527420..6527872	+	453	WP_013396645.1	helix-turn-helix domain-containing protein	-
AXYL_RS30000	6528055..6528366	+	312	WP_013396646.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXYL_RS30005	6528368..6528658	+	291	WP_013396647.1	helix-turn-helix domain-containing protein	Antitoxin
AXYL_RS30020	6530204..6530764	-	561	WP_013396648.1	hypothetical protein	-
AXYL_RS30025	6531135..6533048	+	1914	WP_013396649.1	phosphomethylpyrimidine synthase ThiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	6528775..6529602	827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-75)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11515.39 Da
• Isoelectric Point: 5.0788

>T24959 WP_013396646.1 NC_014640:6528055-6528366 [Achromobacter xylosoxidans A8]
MVFIETSVFTRQITSLLPDDGYRELQQMLLEDPACGNVIPGGGGIRKMRYARPGIGKSGGLRVIYYWLTDDDQILMLLAY
PKSVRDNLSPAEIAELRALVKDL

Nucleotide

• Length: 312 bp

>T24959 NC_014640:6528055-6528366 [Achromobacter xylosoxidans A8]
ATGGTTTTCATCGAAACCAGCGTCTTTACCAGGCAAATCACATCCCTGCTCCCAGATGATGGCTATCGAGAATTGCAGCA
AATGCTGCTGGAGGATCCAGCATGCGGCAATGTGATTCCTGGTGGAGGCGGAATTCGGAAGATGCGCTACGCTCGGCCGG
GCATAGGCAAAAGCGGTGGGCTACGGGTGATCTATTACTGGCTCACCGATGACGACCAGATTCTGATGTTGCTGGCCTAC
CCCAAGAGCGTACGGGACAACTTGAGTCCAGCGGAAATCGCTGAACTGCGGGCGCTGGTAAAGGATCTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10641.34 Da
• Isoelectric Point: 11.3209

>AT24959 WP_013396647.1 NC_014640:6528368-6528658 [Achromobacter xylosoxidans A8]
MDKQLFADLKASLKEAAAIRKGETRPSRVSTLDPLDVKQVREQTKLSQAEFAAALQVSVRTLQNWEQRRRVPTGPAAALL
KVVANAPEIAIRALHR

Nucleotide

• Length: 291 bp

>AT24959 NC_014640:6528368-6528658 [Achromobacter xylosoxidans A8]
ATGGACAAGCAGTTGTTCGCCGATTTGAAGGCAAGCCTCAAAGAGGCTGCAGCGATACGCAAGGGCGAAACACGCCCCTC
GCGGGTATCCACGCTTGATCCTTTGGATGTAAAGCAGGTGCGCGAACAGACCAAGCTGTCTCAGGCCGAATTTGCGGCGG
CGCTGCAGGTCAGCGTTCGCACTTTGCAAAACTGGGAGCAGAGACGCCGAGTGCCTACCGGCCCCGCCGCCGCGCTGCTG
AAAGTCGTGGCCAACGCGCCTGAGATCGCAATACGCGCCTTGCATCGTTAG

Structures

Toxin

• AlphaFold DB: E3HLL6